Cellular stress

Laboratory of Genome Integrity
Senior research group

Cellular stress pathways are essential for cancer cells' survival and significantly contribute to therapeutic resistance. They help the cells to deal with various cancer hallmarks, including elevated DNA damage accompanied by accumulation of mutations, DNA replication problems, accumulation of defective proteins, free radicals, nutrient deficiency, hypoxia, etc. Our methods and expertise identify and target these stress pathways directly inside cancer cells. Such research has great potential in finding new prediction markers, improving standard anticancer therapy, and suggesting new therapeutic targets.

  • DNA damage and repair and genomic instability
  • Replication stress
  • Proteotoxic stress and proteostasis
  • Mechanical stress
  • Oncogenic and free radicals stress
  1. Exploration of mechanical stress-promoted perturbation of the cell cycle in cancer cells.
  2. Identification and structural characterization of novel NPL4 inhibitors and insight into its mode of action.
  3. Cannabidiol as a potential attenuator of the efficacy of chemotherapeutics.
  4. Development of nano-formulated diethyldithiocarbamate-copper complex as an anticancer drug.
  5. Identification of new compounds with senolytic effect and mechanism of their action in senescent cells.
  6. Insight into cellular proteotoxic stress response using new microthermal protein damage methodology.
  7. Effects of long-term mild hypothermia (fever-like hyperthermia) on cancer cells.
  8. Cellular DNA damage response in the context of cance.
ŠKROTT, Z., M. MISTRÍK, K. ANDERSEN, S. FRIIS, D. MAJERA, J. GURSKÝ, T. OŽDIAN, J. BARTKOVA, Z. TURI, P. MOUDRÝ, M. KRAUS, M. MEDVEDÍKOVÁ, J. VÁCLAVKOVÁ, P. DŽUBÁK, I. VROBEL, P. POUCKOVA, J. SEDLACEK, A. MIKLOVICOVA, A. KUTT, J. MATTOVA, C. DRIESSEN, Q. DOU, J. OLSEN, M. HAJDÚCH, B. CVEK, R. DESHAIES, J. BÁRTEK
Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4. Nature. 2017, 552(7684), 194-199, ISSN: 0028-0836, PMID: 29211715,
KOMIS, G., M. MISTRÍK, O. SAMAJOVA, M. OVECKA, J. BÁRTEK, J. SAMAJ
Superresolution live imaging of plant cells using structured illumination microscopy. Nature Protocols. 2015, 10(8), 1248-1263, ISSN: 1754-2189, PMID: 26203822,
BIOAVAILABLE DITHIOCARBAMATE-METAL COMPLEX NANOPARTICLES, METHOD OF PREPARATION AND USE THEREOF "DISULFIRAM I" (Škrott)
  1. Patent: EP 3459526. Granted: 24.2.2021. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  2. Published Application: PCT/EP2018/076098 under WO 2019/063601 (4.4.2019)
  3. Patent: US 11,766,404. Granted: 26.9.2023. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  4. Patent: AU 2018340510. Granted: 5.8.2021. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  5. Published Application: EP 18774051.9 (29.7.2020) - patent pending.
  6. Patent: IL 273652. Granted: 2.1.2024. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  7. Patent: CA 3,076,855. Granted: 26.4.2022. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  8. Patent: KR 10-2456683. Granted: 14.10.2022. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  9. Patent: JP 7149624. Granted: 29.9.2022. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  10. Patent: MX 396,552. Granted: 17.10.2022. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.
  11. Patent:  BR 112020005823-8. Granted: 12.11.2024. Ownership: Palacký University Olomouc. Inventors: Škrott Zdeněk, Mistrík Martin, Hajdúch Marián, Bártek Jiří, Džubák Petr, Zbořil Radek.

Status: Available

METHOD OF OBTAINING FOLLICULAR CELLS AND A DEVICE FOR CARRYING OUT THIS METHOD (Mistrík)
  1. Patent: CZ 304255. Granted: 18.12.2013. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří
  2. Published Application PCT/CZ2013/000152 under WO 2014/086324 (12.6.2014)
  3. Patent: EP 2928382. Granted: 26.12.2018. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří
  4. Patent: US 9,943,331. Granted: 17.4.2018. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří
  5. EU Registered Trademark: EUTM 018714735. Registered: 8.11.2022. Ownership: Palacký University Olomouc

Status: Available

Project: Cellular stress in health and disease
Supervisors: Mistrík Martin Ph.D., Moudrý Pavel Ph.D., Škrott Zdeněk Ph.D.
Available: 3
Intended for: Doctoral training

Group members

BACHELOR STUDENT, LIG
DOCTORAL STUDENT, EXSTAFF, LIG, MASTER STUDENT
IMTM, LIG, STAFF
IMTM, LIG, STAFF
BACHELOR STUDENT, LIG
IMTM, LIG, STAFF
DOCTORAL STUDENT, IMTM, LIG, STAFF
EXSTAFF, LIG, POSTDOCTORAL FELLOW
IMTM, LIG, STAFF
BACHELOR STUDENT, LIG, MASTER STUDENT
IMTM, LIG, POSTDOCTORAL FELLOW, STAFF