Koberna Karel CSc.
O mně
EDUCATION
1989 – 1994 Title CSc. (equivalent of PhD), Institute of Experimental Medicine AS CR
1983 – 1988 Title MSc. and RNDr. (Rerum naturalium doctor), Faculty of Science, Charles University in Prague; Specialization – Molecular Biology
Area of interest, the basic used technologies and special knowledge
- The effect of the precursors of nucleic acids on their metabolism and cellular metabolism, the development of the new methods for the detection of proliferation activity of mammalian cells and systems for the fast detection of proteins based on the magnetic separations, organisation of chromatin, DNA replication and repair, the development of the new methods for measurement of enzyme activity, study of base excision repair and its use in cancer treatment.
- Immunocytochemical detections, confocal and light microscopy, molecular biology methods, cell cultivation, electron microscopy including electron tomography, high pressure freezing and freeze substitution.
- Other knowledge: work with Microsoft Office, Adobe Photoshop, Corel Draw, ImageJ, Cell Profiler, ilastik, 3D model programs: Rhinoceros, programming in Python.
PUBLICATIONS
- Ligasová A., et al. (2023) Int J Mol Sci 24(4): 3674
- Hodoň J., et al. (2022) Eur J Med Chem 243: 114777
- Ligasová A., et al. (2021) Open Biol 11(10): 210136
- Ligasová A., Koberna K. (2021) Int J Mol Sci 22(19): 10759
- Ligasová A., Koberna K. (2021) Molecules 26(18): 5515
- Ligasová A., Koberna K. (2021) Int J Mol Sci 22(13): 7098
- Ligasová A., Koberna K. (2021) Chapter 17, pp 111-120, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
- Ligasová A., Koberna K. (2021) Chapter 18, pp 121-132, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
- Ligasová A., et al. (2019) Cells, 8(12): E1510
- Ligasová A., Koberna K. (2019) Sci. Rep., 9(1): 8701
- Ligasová A., Koberna K. (2018) Molecules, 23(11): 3007
- Ligasová, A., et al. (2017) PLoS One 12(4): e0175880
- Ligasová, A., et al. (2017) PLoS One 12(3): e0174893
- Ligasová, A., et al. (2016) Open Biol, 6:150172
- Ligasová, A., Koberna, K. (2015) Methods Mol. Biol., 1351:81-92, M. McKenzie ed.
- Ligasová, A., et al. (2015) PLoS One 10(7): e0132393
- Ligasová, A., et al. (2015) PLoS One, 10(2): e0117459
- Cmarko, D., et al. (2014) Methods Mol. Biol., 1117:343-66, John Kuo ed.
- Ligasová, A., et al. (2013) PLoS One, 8(6):e66864
- Ligasová, A., et al. (2012) PLoS One, 7(12):e52584
- Liboska, R., et al. (2012) PLoS One, 7(12):e51679
- Ligasová, A., et al. (2011) Parasit. Vectors, 4:162
- Ligasová, A., Koberna, K. (2011) DNA replication-Current Advances, Intech, H. Seligmann ed.
- Ligasová, A., Koberna K. (2010) Nucleic Acids Res., 38(16):e167
- Ligasová, A., et al. (2009) J. Struct. Biol., 165(3):204-213
- Cvačková, Z., et al. (2008) Chromosoma, 117(5):487-497
- Kalmárová, M., et al. (2007) J. Struct. Biol., 160(1):49-56
- Cmarko, D., Koberna, K. (2007) Methods Mol. Biol. 369:213-228, J. Kuo ed.
- Stavreva, D. A., et al. (2006) Mol. Cell Biol., 26:5131-5145
- Smirnov, E., et al. (2006) Folia Biol., 52:59-70
- Fidlerová ,H., et al. (2005) J. Cell Biochem., 94:899-916
- Pliss, A., et al. (2005) J. Cell Biochem., 15:554-565
- Li, J., et al. (2005) EMBO J., 12:120-127
- Koberna, K., et al. (2005) J. Cell Biochem., 94(1):126-138
- Večeřová, J., et al. (2004) Mol. Biol. Cell, 15:4904-4910
- Raška, I., et al. (2004) Biol. Cell, 96:579-594
- Malínský, J., et al. (2002) J. Struct. Biol., 140:227-231
- Koberna, K., et al. (2002) J. Cell Biol., 157:743-748
- Kopský, V., et al. (2002) Fol. Biol. (Prague), 48:69-72
- Staněk, D., et al. (2001) Chromosoma, 110(7):460-470
- Malínský, J., et al. (2001) J. Cell Sci., 114(Pt 4):747-750
- Koberna, K., et al., (2000) Acta Histochem., 102(1):15-20
- Melčák, I., et al. (2000) Mol. Biol. Cell., 11(2):497-510
- Koberna, K., et al. (1999) Chromosoma, 108(5):325-335
- Koberna, K., et al. (1998) Reprod. Nutr. Dev., 38(1):117-126
- Schul, W., et al. (1996) EMBO J., 15(11):2883-2892
- Raška, I., et al. (1995) J. Struct. Biol., 114(1):1-22
- Raška, I., et al. (1992) Cell Biol. Int. Rep., 16(8):771-789
- Raška, I., et al. (1989) Exp. Cell Res., 184(1):81-89
- Raška, I., et al. (1987) Histochem. J., 19(10-11):603
PATENTS
- Czech: 302613; 303230; 303251; 303252; 306187; 307016; 307807; 307304; 307454; 307415; 308519; 308385; 308387, 309218
- European: 3186387, 3395956, 3558526
UTILITY MODELS
- Czech: 28964; 34386; 34442
INDUSTRIAL DESIGN
- Czech: 3668
HONOURS AND AWARDS
2003 Premium of Otto Wichterle for young scientists AS CR
Journals
Books & book chapters
Intellectual property
Utility Model: CZ 34442. Granted: 6.10.2020. Ownership: Palacký University Olomouc. Inventors: Koberna Karel, Ligasová Anna.
Status: Available
Utility Model CZ 34386. Granted: 15.9.2020. Ownership: Palacký University Olomouc. Inventors: KOBERNA Karel, LIGASOVÁ Anna.
Status: Available
Patent: CZ 307304. Granted: 11.4.2018. Ownership: Palacký University Olomouc. Inventors: KOBERNA Karel, LIGASOVÁ Anna
Status: Terminated
Master mentorship
Zálešáková Zuzana
Testing and optimization of new non-destructive method for determining the activity of cytidine deaminase for use of various cell lines
Bachelor mentorship
Pasecká Martina
Optimization of the method for determining the activity of uracil glycosylase and its application to compare the activity of uracil glycosylase in different cell lines
Kociánová Markéta
Metabolic pathways of deoxycytidine and their impact on the cancer treatment by deoxycytidine analog