Koberna Karel CSc.

EDUCATION

1989 – 1994     Title CSc. (equivalent of PhD), Institute of Experimental Medicine AS CR

1983 – 1988     Title MSc. and RNDr. (Rerum naturalium doctor), Faculty of Science, Charles University in Prague; Specialization – Molecular Biology

Area of interest, the basic used technologies and special knowledge

- The effect of the precursors of nucleic acids on their metabolism and cellular metabolism, the development of the new methods for the detection of proliferation activity of mammalian cells and systems for the fast detection of proteins based on the magnetic separations, organisation of chromatin, DNA replication and repair, the development of the new methods for measurement of enzyme activity, study of base excision repair and its use in cancer treatment.

- Immunocytochemical detections, confocal and light microscopy, molecular biology methods, cell cultivation, electron microscopy including electron tomography, high pressure freezing and freeze substitution.

- Other knowledge: work with Microsoft Office, Adobe Photoshop, Corel Draw, ImageJ, Cell Profiler, ilastik, 3D model programs: Rhinoceros, programming in Python.

PUBLICATIONS

1. Ligasová A., et al. (2025) Int J Mol Sci 26(7): 3344
2. Ligasová A., et al. (2025) Sci. Rep. 15(1): 3026
3. Ligasová A., et al. (2023) Sci. Rep. 13: 20530
4. Ligasová A., et al. (2023) Int J Mol Sci 24(4): 3674
5. Hodoň J., et al. (2022) Eur J Med Chem 243: 114777
6. Ligasová A., et al. (2021) Open Biol 11(10): 210136
7. Ligasová A., Koberna K. (2021) Int J Mol Sci 22(19): 10759
8. Ligasová A., Koberna K. (2021) Molecules 26(18): 5515
9. Ligasová A., Koberna K. (2021) Int J Mol Sci 22(13): 7098
10. Ligasová A., Koberna K. (2021) Chapter 17, pp 111-120, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
11. Ligasová A., Koberna K. (2021) Chapter 18, pp 121-132, In Laboratory Techniques in Cellular and Molecular Medicine, Agrawal, K., Bouchal, J., Das, V., Drábek, J., Džubák, P., Hajdúch, M., Koberna, K., Ligasová, A., Mistrík, M., de Sanctis, J.B., et al., Eds. Palacký University Olomouc: Olomouc.
12. Ligasová A., et al. (2019) Cells, 8(12): E1510
13. Ligasová A., Koberna K. (2019) Sci. Rep., 9(1): 8701
14. Ligasová A., Koberna K. (2018) Molecules, 23(11): 3007
15. Ligasová, A., et al. (2017) PLoS One 12(4): e0175880
16. Ligasová, A., et al. (2017) PLoS One 12(3): e0174893
17. Ligasová, A., et al. (2016) Open Biol, 6:150172
18. Ligasová, A., Koberna, K. (2015) Methods Mol. Biol., 1351:81-92, M. McKenzie ed.
19. Ligasová, A., et al. (2015) PLoS One 10(7): e0132393
20. Ligasová, A., et al. (2015) PLoS One, 10(2): e0117459
21. Cmarko, D., et al. (2014) Methods Mol. Biol., 1117:343-66, John Kuo ed.
22. Ligasová, A., et al. (2013) PLoS One, 8(6):e66864
23. Ligasová, A., et al. (2012) PLoS One, 7(12):e52584
24. Liboska, R., et al. (2012) PLoS One, 7(12):e51679
25. Ligasová, A., et al. (2011) Parasit. Vectors, 4:162
26. Ligasová, A., Koberna, K. (2011) DNA replication-Current Advances, Intech, H. Seligmann ed.
27. Ligasová, A., Koberna K. (2010) Nucleic Acids Res., 38(16):e167
28. Ligasová, A., et al. (2009) J. Struct. Biol., 165(3):204-213
29. Cvačková, Z., et al. (2008) Chromosoma, 117(5):487-497
30. Kalmárová, M., et al. (2007) J. Struct. Biol., 160(1):49-56
31. Cmarko, D., Koberna, K. (2007) Methods Mol. Biol. 369:213-228, J. Kuo ed.
32. Stavreva, D. A., et al. (2006) Mol. Cell Biol., 26:5131-5145
33. Smirnov, E., et al. (2006) Folia Biol., 52:59-70
34. Fidlerová ,H., et al. (2005) J. Cell Biochem., 94:899-916
35. Pliss, A., et al. (2005) J. Cell Biochem., 15:554-565
36. Li, J., et al. (2005) EMBO J., 12:120-127
37. Koberna, K., et al. (2005) J. Cell Biochem., 94(1):126-138
38. Večeřová, J., et al. (2004) Mol. Biol. Cell, 15:4904-4910
39. Raška, I., et al. (2004) Biol. Cell, 96:579-594
40. Malínský, J., et al. (2002) J. Struct. Biol., 140:227-231
41. Koberna, K., et al. (2002) J. Cell Biol., 157:743-748
42. Kopský, V., et al. (2002) Fol. Biol. (Prague), 48:69-72
43. Staněk, D., et al. (2001) Chromosoma, 110(7):460-470
44. Malínský, J., et al. (2001) J. Cell Sci., 114(Pt 4):747-750
45. Koberna, K., et al., (2000) Acta Histochem., 102(1):15-20
46. Melčák, I., et al. (2000) Mol. Biol. Cell., 11(2):497-510
47. Koberna, K., et al. (1999) Chromosoma, 108(5):325-335
48. Koberna, K., et al. (1998) Reprod. Nutr. Dev., 38(1):117-126
49. Schul, W., et al. (1996) EMBO J., 15(11):2883-2892
50. Raška, I., et al. (1995) J. Struct. Biol., 114(1):1-22
51. Raška, I., et al. (1992) Cell Biol. Int. Rep., 16(8):771-789
52. Raška, I., et al. (1989) Exp. Cell Res., 184(1):81-89
53. Raška, I., et al. (1987) Histochem. J., 19(10-11):603

PATENTS                                      

• Czech: 302613; 303230; 303251; 303252; 306187; 307016; 307807; 307304; 307454; 307415; 308519; 308385; 308387, 309218
• European: 3186387, 3395956, 3558526

UTILITY MODELS

• Czech: 28964; 34386; 34442; 38478

INDUSTRIAL DESIGN

• Czech: 3668

HONOURS AND AWARDS

2003     Premium of Otto Wichterle for young scientists AS CR