Friedecký David Ph.D.

Phone: +420588442619
Curriculum Vitae: download here

Senior Researcher at IMTM

1) Analytical chemistry - separation techniques
    - liquid chromatography
    - capillary electrophoresis
    - mass spectrometry
    - flow injection analysis / MS

2) Inherited Metabolic Disorders
    - purine and pyrimidine metabolism
    - expanded newborn screening
    - LC/MS methods for diagnosing IMD

3) Therapeutic drug monitoring
    - tyrosine kinase inhibitors
    - immunosuppresants
    - antihypertensives
    - vitamin D3
    - methotrexate

4) Metabolomics
    - LC/MS, GC/MS, FIA/MS
    - Quadrupole analyzers, Orbitrap
    - data processing, interpretation
    - analysis of buifluids, cells...

Specialties: Development mass spectrometry methods for diagnosing IMD, TDM and high throughput analysis by FIA/MS
Development and application of metabolomics in medicine



1. prize in The national student competition of scientific works in the analytical chemistry section, Praha, 1998.

1. prize - dean´s award for the best student work in the chemistry - Student conference, Olomouc, 1999.

TALENT ´98 - Honorable mention of Minister of Education, Youth and Sports for works in the field of analysis of purine and pyrimidine compounds - Praha, 1999.

Vice-Chancellor´s award for the best student scientific work - Olomouc, 1999.

1. prize - award for the best lecture in section of young clinical biochemists - FONS 2000, Pardubice.

Award of club of alumnuses and friends of UP for pregraduate and postgraduate students for scientisic articel - 2001.

Publications (Impact Factor Journals)

  • [34]
    KOUŘIL, ?.., J. DE SOUSA, J. VÁCLAVÍK, D. FRIEDECKÝ a T. ADAM. CROP: Correlation-based reduction of feature multiplicities in untargeted metabolomic data. Bioinformatics (Oxford, England). 2020, -, -. ISSN 1367-4803. IF: 4.531. PMID: 31930393
  • [33]
    DE SOUSA, J., O. VENCALEK, K. HRON, J. VÁCLAVÍK, D. FRIEDECKÝ a T. ADAM. Bayesian multiple hypotheses testing in compositional analysis of untargeted metabolomic data. Analytica Chimica Acta. 2020, 1097, 49-61. ISSN 0003-2670. IF: 5.256. PMID: 31910969
  • [32]
    JÁČOVÁ, J., M. JORENEK, K. POSPISKOVA, L. NAJDEKR, L. ZAJONCOVA, D. FRIEDECKÝ a T. ADAM. Urease-immobilized magnetic microparticles in urine sample preparation for metabolomic analysis by gas chromatography-mass spectrometry. Journal of Chromatography. A. 2019, 1605, 360355. ISSN 0021-967. IF: 3.858. PMID: 31315811
  • [31]
    HOEVENAARS, F., J. KEIJER, I. VAD DER STELT, L. DUIVENVOORDE, L. HERREMAN, R. VAN NES, D. FRIEDECKÝ, M. HEGEMAN a E. VAN SCHOTHORST. White Adipose Tissue Response of Obese Mice to Ambient Oxygen Restriction at Thermoneutrality: Response Markers Identified, but no WAT Inflammation. Genes. 2019, 10(5), pii: E359. ISSN 2073-4425. IF: 3.191. PMID: 31083422
  • [30]
    MÁDROVÁ, L., M. KRIJT, V. BARESOVA, J. VÁCLAVÍK, D. FRIEDECKÝ, D. DOBEŠOVÁ, O. SOUCKOVA, V. SKOPKOVA, T. ADAM a M. ZIKANOVA. Mass spectrometric analysis of purine de novo biosynthesis intermediates. PLOS One. 2018, 13(12), e0208947. ISSN 1932-6203. IF: 2.766. PMID: 30532129
  • [29]
    KUČERA, J., Z. SPACIL, D. FRIEDECKÝ, J. NOVAK, M. PEKAR a J. BIENERTOVA-VASKU. Human White Adipose Tissue Metabolome: Current Perspective. Obesity. 2018, 26(12), 1870-1878. ISSN 1930-7381. IF: 4.042. PMID: 30369078
  • [28]
    KARLÍKOVÁ, R., J. ŠIROKÁ, M. MECH, D. FRIEDECKÝ, H. JANEČKOVÁ, L. MÁDROVÁ, F. HRDINOVA, Z. DRABKOVA, O. DOBESOVA, T. ADAM a P. JAHN. Newborn foal with atypical myopathy. Journal of Veterinary Internal Medicine. 2018, 32(5), 1768-1772. ISSN 0891-6640. IF: 2.185. PMID: 30216546
  • [27]
    SALOVSKA, B., H. JANEČKOVÁ, I. FABRIK, R. KARLÍKOVÁ, L. CECHAKOVA, M. ONDREJ, M. LINK, D. FRIEDECKÝ a A. TICHY. Radio-sensitizing effects of VE-821 and beyond: Distinct phosphoproteomic and metabolomic changes after ATR inhibition in irradiated MOLT-4 cells. PLOS One. 2018, 13(7), e0199349. ISSN 1932-6203. IF: 2.766. PMID: 30001349
  • [26]
    VÁCLAVÍK, J., K. COENE, I. VROBEL, L. NAJDEKR, D. FRIEDECKÝ, R. KARLÍKOVÁ, L. MÁDROVÁ, A. PETSALO, U. ENGELKE, A. VAN WEGBERG, L. KLUIJTMANS, T. ADAM a R. WEVERS. Structural elucidation of novel biomarkers of known metabolic disorders based on multistage fragmentation mass spectra. Journal of Inherited Metabolic Disease. 2018, 41(3), 407-414. ISSN 0141-8955. IF: 3.970. PMID: 29139026
  • [25]
    KARLÍKOVÁ, R., K. MIČOVÁ, L. NAJDEKR, A. GARDLO, T. ADAM, P. MAJEROVÁ, D. FRIEDECKÝ a A. KOVAC. Metabolic status of CSF distinguishes rats with tauopathy from controls. Alzheimer's Research & Therapy. 2017, 9(1), 78. ISSN 1758-9193. IF: 6.154. PMID: 28934963
  • [24]
    CAMATEROS, P., C. KANAGARATHAM, L. NAJDEKR, D. HOLUB, J. VRBKOVÁ, L. COTE, J. FOURNIER, J. GOURDON, D. CREERY, R. OLIVENSTEIN, F. KOPŘIVA, T. ADAM, D. FRIEDECKÝ, P. DŽUBÁK, M. HAJDÚCH a D. RADZIOCH. Toll-Like Receptor 7/8 Ligand, S28463, Suppresses Ascaris Suum Induced Allergic Asthma in Nonhuman Primates. American Journal of Respiratory Cell and Molecular Biology. 2018, 58(1), 55-65. ISSN 1044-1549. IF: 4.100. PMID: 28850259
  • [23]
    JÁČOVÁ, J., A. GARDLO, D. FRIEDECKÝ, T. ADAM a J. DIMANDJA. Sample-independent approach to normalize two-dimensional data for orthogonality evaluation using whole separation space scaling. Journal of Chromatography. A. 2017, 1511(2017), 1-8. ISSN 0021-9673. IF: 3.981. PMID: 28693825
  • [22]
    VROBEL, I., D. FRIEDECKÝ, E. FABER, L. NAJDEKR, K. MIČOVÁ, R. KARLÍKOVÁ a T. ADAM. Novel sulphur-containing imatinib metabolites found by untargeted LC-HRMS analysis. European Journal of Pharmaceutical Sciences. 2017, -, -. ISSN 0928-0987. IF: 3.773. PMID: 28433749
  • [21]
    VAD DER STELT, I., F. HOEVENAARS, J. ŠIROKÁ, J. DE RONDE, D. FRIEDECKÝ, J. KEIJER a E. VAN SCHOTHORST. Metabolic Response of Visceral White Adipose Tissue of Obese Mice Exposed for 5 Days to Human Room Temperature Compared to Mouse Thermoneutrality. Frontiers in Physiology. 2017, 8:179, doi: 10.3389/fphys.2017.00179. ISSN 1664-042X. IF: 4.031. PMID: 28386236
  • [20]
    NAJDEKR, L., D. FRIEDECKÝ, R. TAUTENHAHN, T. PLUSKAL, J. WANG, Y. HUANG a T. ADAM. Influence of Mass Resolving Power in Orbital Ion-Trap Mass Spectrometry-Based Metabolomics. Analytical Chemistry. 2016, 88(23), 11429-11435. ISSN 0003-2700. IF: 6.320. PMID: 27809493
  • [19]
    KARLÍKOVÁ, R., J. ŠIROKÁ, P. JAHN, D. FRIEDECKÝ, A. GARDLO, H. JANEČKOVÁ, F. HRDINOVA, Z. DRABKOVA a T. ADAM. Equine atypical myopathy: A metabolic study. Veterinary Journal. 2016, 216(2016), 125-132. ISSN 1090-0233. IF: 1.802. PMID: 27687939
  • [18]
    KARLÍKOVÁ, R., J. ŠIROKÁ, D. FRIEDECKÝ, E. FABER, M. HRDA, K. MIČOVÁ, I. FIKAROVA, A. GARDLO, H. JANEČKOVÁ, I. VROBEL a T. ADAM. Metabolite Profiling of the Plasma and Leukocytes of Chronic Myeloid Leukemia Patients. Journal of Proteome Research. 2016, 15(9), 3158-66. ISSN 1535-3893 . IF: 4.268. PMID: 27465658
  • [17]
    VROBEL, I., H. JANEČKOVÁ, E. FABER, K. BOUCHALOVÁ, K. MIČOVÁ, D. FRIEDECKÝ a T. ADAM. Ultrafast Online SPE-MS/MS Method for Quantification of 3 Tyrosine Kinase Inhibitors in Human Plasma. Therapeutic Drug Monitoring. 2016, 38(4), 516-524. ISSN 0163-4356 . IF: 2.178. PMID: 27414975
  • [16]
    LIGASOVÁ, A., R. LIBOSKA, D. FRIEDECKÝ, K. MIČOVÁ, T. ADAM, T. OŽDIAN, I. ROSENBERG a K. KOBERNA. Dr Jekyll and Mr Hyde: A Strange Case of 5-Ethynyl-2'-deoxyuridine and 5-ethynyl-2'-deoxycytidine. Open Biology. 2016, 6: 150172, 1-11. ISSN 2046-2441. IF: 3.481. PMID: 26740587
  • [15]
    FRIEDECKÝ, D., K. MIČOVÁ, E. FABER, M. HRDA, J. ŠIROKÁ a T. ADAM. Detailed study of imatinib metabolization using high-resolution mass spectrometry. Journal of Chromatography. 2015, 1409(-), 173-181. ISSN 0021-9673. IF: 3.926. PMID: 26199102
  • [14]
    ZEZULOVÁ, M., M. BARTOUŠKOVÁ, E. HLIDKOVA, T. ADAM, L. KUJOVSKA KRCMOVA, B. CERVINKOVA, D. SOLICHOVA, M. ZLEVOROVA, K. CWIERTKA, D. FRIEDECKÝ, D. VRÁNA a B. MELICHAR. Citrulline as a biomarker of gastrointestinal toxicity in patients with rectal carcinoma treated with chemoradiation. Clinical Chemistry and Laboratory Medicine . 2015, 54(2), 305-314. ISSN 1434-6621 . IF: 3.017. PMID: 26167980
  • [13]
    NAJDEKR, L., A. GARDLO, L. MÁDROVÁ, D. FRIEDECKÝ, H. JANEČKOVÁ, E. CORREA, R. GOODACRE a T. ADAM. Oxidized phosphatidylcholines suggest oxidative stress in patients with medium-chain acyl-CoA dehydrogenase deficiency. Talanta. 2015, 139(2015), 62-66. ISSN 0039-9140. IF: 4.035. PMID: 25882409
  • [12]
    LIGASOVÁ, A., D. STRUNIN, D. FRIEDECKÝ, T. ADAM a K. KOBERNA. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity. PLOS One. 2015, 10(2), 1-21. ISSN 1932-6203. IF: 3.057. PMID: 25671308
  • [11]
    JANEČKOVÁ, H., A. KALIVODOVÁ, L. NAJDEKR, D. FRIEDECKÝ, K. HRON, P. BRUHEIM a T. ADAM. Untargeted metabolomic analysis of urine samples in the diagnosis of some inherited metabolic disorders. Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic. 2015, 159(4), 582-585. ISSN 1213-8118. IF: 0.924. PMID: 25482736
  • [10]
    KANAGARATHAM, C., A. KALIVODOVÁ, L. NAJDEKR, D. FRIEDECKÝ, T. ADAM, M. HAJDÚCH, J.B. DE SANCTIS a D. RADZIOCH. Fenretinide prevents inflammation and airway hyperresponsiveness in a mouse model of allergic asthma. American Journal of Respiratory Cell and Molecular Biology. 2014, 51(6), 783-92. ISSN 1535-4989. IF: 3.985. PMID: 24885263
  • [9]
    ČÍŽKOVÁ, M., K. BOUCHALOVÁ, D. FRIEDECKÝ, A. POLYNKOVA, A. JANOŠŤÁKOVÁ, L. RADOVÁ, K. CWIERTKA, R. TROJANEC, M. ZEZULOVÁ, M. ZLEVOROVA, M. HAJDÚCH a B. MELICHAR. High lapatinib plasma levels in breast cancer patients: risk or benefit?. Tumori. 2012, 98(1), 162-165. ISSN 0300-8916. IF: 0.922. PMID: 22495718
  • [8]
    MIČOVÁ, K., D. FRIEDECKÝ, E. FABER a T. ADAM. Isotope dilution direct injection mass spectrometry method for determination of four tyrosine kinase inhibitors in human plasma. Talanta. 2012, 93(-), 307-317. ISSN 0039-9140. IF: 3.498. PMID: 22483915
  • [7]
    FABER, E., D. FRIEDECKÝ, K. MIČOVÁ, S. ROZMANOVA, M. DIVOKÁ, M. JAROSOVA, K. INDRAK a T. ADAM. Imatinib trough plasma levels do not correlate with the response to therapy in patients with chronic myeloid leukemia in routine clinical setting. Annals of Hematology. 2012, 91(6), 923-929. ISSN 0939-5555. IF: 2.866. PMID: 22231281
  • [6]
    JANEČKOVÁ, H., K. HRON, P. WOJTOWICZ, E. HLIDKOVA, A. BARESOVA, D. FRIEDECKÝ, L. ŽÍDKOVÁ, P. HORNIK, D. BEHULOVA, D. PROCHAZKOVA, H. VINOHRADSKA, K. PESKOVA, P. BRUHEIM, V. SMOLKA, S. STASTNA a T. ADAM. Targeted metabolomic analysis of plasma samples for the diagnosis of inherited metabolic disorders. Journal of Chromatography A. 2012, 1226(-), 11-17. ISSN 1873-3778. IF: 4.612. PMID: 22018716
  • [5]
    BOUCHALOVÁ, K., M. ČÍŽKOVÁ, K. CWIERTKA, R. TROJANEC, D. FRIEDECKÝ a M. HAJDÚCH. Lapatinib in breast cancer - the predictive significance of HER1 (EGFR), HER2, PTEN and PIK3CA genes and lapatinib plasma level assessment. Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic. 2010, 154(4), 281-288. ISSN 1213-8118. IF: 0.716. PMID: 21293538
  • [4]
    ŽÍDKOVÁ, L., J. KRIJT, J. SLADKOVA, A. HLOBILKOVA, M. MAGNER, M. ZIKANOVA, S. KMOCH, D. FRIEDECKÝ, J. ZEMAN, M. ELLEDER a T. ADAM. Oligodendroglia from ADSL-deficient patient produce SAICAribotide and SAMP. Molecular Genetics and Metabolism. 2010, 101(2-3), 286-288. ISSN 1096-7192. IF: 3.539. PMID: 20674424
  • [3]
    WOJTOWICZ, P., H. JANEČKOVÁ, D. FRIEDECKÝ a T. ADAM. Techniky metabolomiky v biomedicíně. Chemické Listy. 2013, 107(1), 3-11. ISSN 0009-2770. IF: 0.196.
  • [2]
    BARESOVA, A., D. FRIEDECKÝ a T. ADAM. Analysis of Nucleotides in Dry Blood Spots. Chemicke Listy. 2011, 105(3), 207-211. ISSN 0009-2770. IF: 0.529.
  • [1]
    GARDLO, A., A. SMILDE, K. HRON, M. HRDA, R. KARLÍKOVÁ, D. FRIEDECKÝ a T. ADAM. Normalization techniques for PARAFAC modeling of urine metabolomic data. Metabolomics. 2016, 12(7), a.n. 117. ISSN 1573-3882. IF: 3.692.
  • Publications (Other Reviewed Journals)

  • [5]
    VROBEL, I., D. FRIEDECKÝ a T. ADAM. Analytický systém online extrakce na pevné fázi ve spojení s tandemovou spektrometrií a jeho klinické využití. Klinická biochemie a metabolismus. 2018, 26(47), 11-17. ISSN 2570-9402.
  • [4]
    FRIEDECKÝ, D. a K. LEMR. Úvod do hmotnostní spektrometrie. Klinická biochemie a metabolismus. 2012, 20(3), 154-159. ISSN 1210-7921.
  • [3]
    FRIEDECKÝ, D. a K. LEMR. Hmotnostní spektrometrie - zdroj analytických informací. Klinická biochemie a metabolismus. 2012, 20(4), 210-215. ISSN 1210-7921.
  • [2]
    JANEČKOVÁ, H., P. WOJTOWICZ, K. HRON, D. FRIEDECKÝ a T. ADAM. Necílená metabolomická analýza suchých krevních skvrn pro diagnostiku dědičných metabolických poruch. Klinická biochemie a metabolismus. 2012, 20(41), 167-169. ISSN 1210-7921.
  • [1]
    MIČOVÁ, K., D. FRIEDECKÝ, E. FABER a T. ADAM. Rutinní terapeutické monitorování tyrosinkinázových inhibitorů metodou LC-MS/MS. Klinická biochemie a metabolismus. 2012, 20(41), 222-225. ISSN 1210-7921.

  • Doctoral students mentor:

    [4] Śtěpán Kouřil, Lipidomic analysis of biological samples by HR-MS,
    Ph.D. specialization: Medical chemistry and clinical biochemistry, status: Ongoing, from: 2017
    [3] Ivo Vrobel, Influence of certain cancerostatics on the cell metabolome,
    Ph.D. specialization: , status: Graduated, from: 2013 to 2018
    [2] Radana Brumarová, Study of patobiochemistry of inherited metabolic disorders by metabolomic approach,
    Ph.D. specialization: , status: Graduated, from: 2013 to 2018
    [1] Kateřina Mičová, Significance of intracellular and plasma levels of new tyrosine kinase inhibitors,
    Ph.D. specialization: , status: Graduated, from: 2009 to 2014

    Master students mentor:

    [3] Aleš Kvasnička, Structural elucidation of novel biomarkers of known inherited metabolic disorders using high resolution mass spectrometry,
    status: Ongoing, from: 2019
    [2] Petra Šťastná, LC-HRMS analysis of plasma samples from patients with chronic myeloid leukemia – study of correlation with clinical response,
    status: Ongoing, from: 2018
    [1] Klára Míčková, Lipidomics for clinical diagnostics,
    status: Ongoing, from: 2017