Cellular stress

Laboratory of Genome Integrity
Senior research group

Cellular stress pathways are essential for cancer cells' survival and significantly contribute to therapeutic resistance. They help the cells to deal with various cancer hallmarks, including elevated DNA damage accompanied by accumulation of mutations, DNA replication problems, accumulation of defective proteins, free radicals, nutrient deficiency, hypoxia, etc. Our methods and expertise identify and target these stress pathways directly inside cancer cells. Such research has great potential in finding new prediction markers, improving standard anticancer therapy, and suggesting new therapeutic targets.

  • DNA damage and repair and genomic instability
  • Replication stress
  • Proteotoxic stress and proteostasis
  • Mechanical stress
  • Oncogenic and free radicals stress
  1. Exploration of mechanical stress-promoted perturbation of the cell cycle in cancer cells.
  2. Identification and structural characterization of novel NPL4 inhibitors and insight into its mode of action.
  3. Cannabidiol as a potential attenuator of the efficacy of chemotherapeutics.
  4. Development of nano-formulated diethyldithiocarbamate-copper complex as an anticancer drug.
  5. Identification of new compounds with senolytic effect and mechanism of their action in senescent cells.
  6. Insight into cellular proteotoxic stress response using new microthermal protein damage methodology.
  7. Effects of long-term mild hypothermia (fever-like hyperthermia) on cancer cells.
  8. Cellular DNA damage response in the context of cance.
CHROMÁ, K., Z. ŠKROTT, J. GURSKÝ, J. BACOVSKY, P. MOUDRÝ, T. BUCHTOVÁ, M. MISTRÍK, J. BÁRTEK
A drug repurposing strategy for overcoming human multiple myeloma resistance to standard-of-care treatment. Cell Death & Disease. 2022, 13(3), 203, ISSN: 2041-4889, PMID: 35246527,
BUCHTOVÁ, T., Z. ŠKROTT, K. CHROMÁ, J. ŘEHULKA, P. DŽUBÁK, M. HAJDÚCH, D. LUKÁČ, S. ARAMPATZIS, J. BÁRTEK, M. MISTRÍK
Cannabidiol-induced activation of the metallothionein pathway impedes anticancer effects of disulfiram and its metabolite CuET. Molecular Oncology. 2021, 16(7), 1541-1554, ISSN: 1574-7891, PMID: 34632694,
MISTRÍK, M., Z. ŠKROTT, P. MULLER, A. PANACEK, L. HOCHVALDOVA, K. CHROMÁ, T. BUCHTOVÁ, V. VANDOVA, L. KVÍTEK, J. BÁRTEK
Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response. Nature Communications. 2021, 12(1), 713, ISSN: 2041-1723, PMID: 33514738,
LIQUID CHROMATOGRAPHY COLUMN (Mistrík)

Utility Model: CZ 35118. Granted: 1.6.2021. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, ŠKROTT Zdeněk, OŽDIAN Tomáš.

Status: Available

REMOVABLE CULTURE KIT FOR CELL CULTURES GROWN ON CULTURE DISHES (Mistrík)

Utility Model: CZ 34546. Granted: 16.11.2020. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří

Status: Available

MOLECULAR COMPLEX ASSEMBLY PARTICLES COMRISING BIS-R1, R2-DITHIOCARBAMATE-METAL COMPLEX AND A LIGAND, METHOD OF PREPARATION AND USE THEREOF "DISULFIRAM II" (Mistrík)
  1. Published Application EP 18199181.1 under EP 3636285 (15.4.2020)
  2. Published Application PCT/EP2019/077222 under WO 2020/074514 (16.4.2020)
  3. Patent: AU 2019358428. Granted: 17.11.2022. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří, ŠKROTT Zdeněk, DŽUBÁK Petr, HAJDÚCH Marián
  4. Patent: CA 3,114,031. Granted: 12.3.2024. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří, ŠKROTT Zdeněk, DŽUBÁK Petr, HAJDÚCH Marián
  5. Patent: IL 281616. Granted: 2.4.2024. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří, ŠKROTT Zdeněk, DŽUBÁK Petr, HAJDÚCH Marián
  6. Patent: JP 7220932. Granted: 3.2.2023. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří, ŠKROTT Zdeněk, DŽUBÁK Petr, HAJDÚCH Marián
  7. Patent: KR 10-2626651. Granted: 17.1.2024. Ownership: Palacký University Olomouc. Inventors: MISTRÍK Martin, BÁRTEK Jiří, ŠKROTT Zdeněk, DŽUBÁK Petr, HAJDÚCH Marián

Status: Patents pending, Available

Project: Cellular stress in health and disease
Supervisors: Mistrík Martin Ph.D., Moudrý Pavel Ph.D., Škrott Zdeněk Ph.D.
Available: 3
Intended for: Doctoral training

Group members

BACHELOR STUDENT, LIG
DOCTORAL STUDENT, EXSTAFF, LIG, MASTER STUDENT
IMTM, LIG, STAFF
IMTM, LIG, STAFF
BACHELOR STUDENT, LIG
IMTM, LIG, STAFF
DOCTORAL STUDENT, IMTM, LIG, STAFF
EXSTAFF, LIG, POSTDOCTORAL FELLOW
IMTM, LIG, STAFF
BACHELOR STUDENT, LIG, MASTER STUDENT
IMTM, LIG, POSTDOCTORAL FELLOW, STAFF