On Wednesday April 23, 2025, East Port held a seminar Bioluminescence in Action: Monitoring Cell Health, Metabolism, and Protein Dynamics in the seminary room 1.09.
This seminar highlights a suite of advanced, luminescence-based technologies for investigating cell health, metabolism, and protein dynamics in cellular systems. Promega’s assay platforms offer sensitive and scalable approaches for monitoring viability, cytotoxicity, apoptosis, oxidative stress, and metabolic function, with applications in cancer research and the study of metabolic diseases.
The NanoLuc® luciferase platform, including the HiBiT Protein Tagging System, enables real-time and endpoint tracking of protein abundance and turnover. With applications in receptor internalization, protein degradation, and immune-mediated cytotoxicity, HiBiT supports precise, antibody-free detection of endogenous or overexpressed proteins.
The Lumit® Immunoassay platform complements these tools with rapid, no-wash quantification of secreted proteins, cytokines, and post-translational markers such as protein phosphorylation and proliferation indicators like the newly available Ki-67 assay. Together, these technologies offer powerful solutions for exploring dynamic biological processes with high specificity and throughput.
In the last part of the seminar, we will introduce you label-free approaches such as live-cell imaging or impedance measurements that can be combined with fluorescent and luminescent assays. This way you can observe and quantify specific markers and also study the morphology of the cells, the junctions between cells and maximize the data output from your precious samples.
10:00–10:50
Dr.rer.nat. Kerem Yildirim
10:50–11:10
Coffee Break
11:10–11:40
Dr. Vojtěch Ledvina
Speakers
Dr.rer.nat. Kerem Yildirim (Promega)
Dr. Kerem Yıldırım provides scientific expertise and supports the implementation of Promega’s innovative solutions and technologies, tailored to the specific goals of each customer’s research. His mission is to empower scientists with the right tools and resources to accelerate their discoveries and achieve impactful outcomes.
Dr. Vojtěch Ledvina (East Port)
Dr. Vojtěch Ledvina is an application specialist for our complete life science portfolio, namely cell assays, reporter assays, molecular biology, proteomics and scientific laboratory instruments. He is also responsible for organizing webinars and seminars introducing our suppliers' technologies, at which he lectures himself.
Photo: Denisa Pavelková
