IMTM’s high throughput screening and high content analysis platform is one of the largest academic installations worldwide and provides screening and high volume biology data on a broad diversity of assays and detection systems. Our HTS platform is industry strong, modular and flexible, and allows testing in BSL3 and BSL2+ environment, screening in combination with ionizing radiation (X-rays), mass spectrometry, high content analysis and others. The screening assays provide leads for downstream drug research and development.
Equipment:
The HTS/HCA screening platform is based on a state-of-art robotic system provided by HighResBiosolutions Ltd. The system consists of three robotic arms, automatic incubators, liquid handlers for microliter and nanoliter volumes, sealers, de-sealers, centrifuges and readers for fluorescence, luminescence, absorbance and ionizing radiation. Also integrated with the system are wide-field or spinning disc confocal microscopes (Operetta, Yokogawa CV7000) equipped with software tools for image analysis and data evaluation. A critical part of the robotic system is the automatized chemical library, which contains more than 110 000 compounds.
Our tests focus on:
Academic and commercial offer:
Collaborative and contractual R&D projects in the field of uHTS/HCA and chemical biology/genetics.
Tests of biological activities of small molecules.
Head of the facility
Soňa Gurská
Bioinformatics
Jiří Voller
Miroslav Hruška