Holub Dušan M.Sc., Ph.D.

Holub Dušan M.Sc., Ph.D.'s picture
Phone: +420 585632068, +420 585632069
Doctoral training
Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LCMS
Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. As such, it has potential as an indicator of any iron-related condition, such as anemia. My research focuses on the measurement of hepcidin in biological fluids to improve our understanding of iron-related disorders. A possible result of such studies could be the development of hepcidin as a tool for diagnosis and clinical management of such disorders.



Publications (Impact Factor Journals):

  • [12]
    KARASKOVA, E., J. VOLEJNÍKOVÁ, D. HOLUB, M. VELGANOVA-VEGHOVA, M. SPENEROVA, V. MIHÁL a D. POSPÍŠILOVÁ. Changes in serum hepcidin levels in children with inflammatory bowel disease during anti-inflammatory treatment. Journal of Paediatrics and Child Health. 2019, -, -. ISSN 1034-4810. IF: 1.688. PMID: 31411363
  • [11]
    KARASKOVA, E., J. VOLEJNÍKOVÁ, D. HOLUB, M. VELGANOVA-VEGHOVA, L. SULOVSKÁ, V. MIHÁL, M. HORVÁTHOVÁ a D. POSPÍŠILOVÁ. Hepcidin in newly diagnosed inflammatory bowel disease in children. Journal of Paediatrics and Child Health. 2018, 54(12), 1362-1367. ISSN 1034-4810. IF: 3.595. PMID: 29923651
  • [10]
    MRAZKOVA, B., R. DZIJAK, T. IMRICHOVA, L. KYJACOVA, P. BARATH, P. DŽUBÁK, D. HOLUB, M. HAJDÚCH, Z. NAHACKA, L. ANDERA, P. HOLICEK, P. VASICOVA, O. SAPEGA, J. BÁRTEK a Z. HODNY. Induction, regulation and roles of neural adhesion molecule L1CAM in cellular senescence. Aging. 2018, 10(3), 432-462. ISSN 1945-4589. IF: 4.867. PMID: 29615539
  • [9]
    FLODROVÁ, P., P. FLODR, T. PIKA, J. VYMETAL, D. HOLUB, P. DŽUBÁK, M. HAJDÚCH a V. SCUDLA. Cardiac amyloidosis: from clinical suspicion to morphological diagnosis. Pathology. 2018, 50(3), 261-268. ISSN 0031-3025. IF: 2.709. PMID: 29448998
  • [8]
    CAMATEROS, P., C. KANAGARATHAM, L. NAJDEKR, D. HOLUB, J. VRBKOVÁ, L. COTE, J. FOURNIER, J. GOURDON, D. CREERY, R. OLIVENSTEIN, F. KOPŘIVA, T. ADAM, D. FRIEDECKÝ, P. DŽUBÁK, M. HAJDÚCH a D. RADZIOCH. Toll-Like Receptor 7/8 Ligand, S28463, Suppresses Ascaris Suum Induced Allergic Asthma in Nonhuman Primates. American Journal of Respiratory Cell and Molecular Biology. 2018, 58(1), 55-65. ISSN 1044-1549. IF: 4.100. PMID: 28850259
  • [7]
    MOJZIKOVA, R., P. KOŘALKOVÁ, D. HOLUB, Z. SAXOVA, D. POSPÍŠILOVÁ, D. PROCHAZKOVA, P. DŽUBÁK, M. HORVÁTHOVÁ a V. DIVOKÝ. Two novel mutations (p.(Ser160Pro) and p.(Arg472Cys)) causing glucose-6-phosphate isomerase deficiency are associated with erythroid dysplasia and inappropriately suppressed hepcidin. Blood cells, molecules & diseases. 2017, 69, 23-29. ISSN 1079-9796. IF: 1.882. PMID: 28803808
  • [6]
    OŽDIAN, T., D. HOLUB, Z. MACEČKOVÁ, L. VARANASI, G. RYLOVÁ, J. ŘEHULKA, J. VÁCLAVKOVÁ, H. SLAVÍK, P. MOUDRÝ, P. ZNOJEK, J. STANKOVÁ, J.B. DE SANCTIS, M. HAJDÚCH a P. DŽUBÁK. Proteomic profiling reveals DNA damage, nucleolar and ribosomal stress are the main responses to oxaliplatin treatment in cancer cells. Journal of Proteomics. 2017, 162, 73-85. ISSN 1874-3919 . IF: 3.867. PMID: 28478306
  • [5]
    SULOVSKÁ, L., D. HOLUB, Z. ŽIDOVÁ, M. DIVOKÁ, M. HAJDÚCH, V. MIHÁL, J. VRBKOVÁ, M. HORVÁTHOVÁ a D. POSPÍŠILOVÁ. Characterization of iron metabolism and erythropoiesis in erythrocyte membrane defects and thalassemia traits. Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic. 2015, 160(2), 231-237. ISSN 1213-8118 . IF: 0.924. PMID: 26592557
  • [4]
    RYLOVÁ, G., T. OŽDIAN, L. VARANASI, M. SOURAL, J. HLAVÁČ, D. HOLUB, P. DŽUBÁK a M. HAJDÚCH. Affinity-Based Methods in Drug-Target Discovery. Current Drug Targets. 2015, 16(1), 60-76. ISSN 1389-4501. IF: 3.029. PMID: 25410410
  • [3]
    POSPÍŠILOVÁ, D., D. HOLUB, Z. ŽIDOVÁ, L. SULOVSKÁ, J. HOUDA, V. MIHÁL, I. HADAČOVÁ, L. RADOVÁ, P. DŽUBÁK, M. HAJDÚCH, V. DIVOKÝ a M. HORVÁTHOVÁ. Hepcidin levels in Diamond-Blackfan anemia reflect erythropoietic activity and transfusion dependency. Haematologica. 2014, 99(7), e118-121. ISSN 0390-6078. IF: 5.814. PMID: 24727814
  • [2]
    MOJZIKOVA, R., P. KORALKOVA, D. HOLUB, Z. ŽIDOVÁ, D. POSPÍŠILOVÁ, J. CERMAK, Z.S. LALUHOVA, K. INDRAK, M. SUKOVA, M. PARTSCHOVA, J. KUCEROVA, M. HORVÁTHOVÁ a V. DIVOKÝ. Iron status in patients with pyruvate kinase deficiency: neonatal hyperferritinaemia associated with a novel frameshift deletion in the PKLR gene (p.Arg518fs), and low hepcidin to ferritin ratios. British Journal of Haematology. 2014, 165(4), 556-563. ISSN 0007-1048. IF: 4.971. PMID: 24533562
  • [1]
    HOLUB, D., P. FLODROVÁ, T. PIKA, P. FLODR, M. HAJDÚCH a P. DŽUBÁK. Mass Spectrometry Amyloid Typing Is Reproducible across Multiple Organ Sites. Biomed Research International. 2019, -, -. ISSN 2314-6133. IF: 2.583.

Publications (Other Reviewed Journals):

Master students mentor:

[1] Lucie Broskevičová, Typing of systemic amyloidosis using mass spectrometry-based proteomic analysis,
status: Graduated, from: 2014 to 2016