Laboratory of Experimental Medicine

Group name

Nanomaterials in medicine

Junior research group

The field of nanomaterials in medicine (often labelled as nanomedicine), covers many types of nanomaterials of various structures or morphologies, and advanced mostly optical or electrochemical techniques optimized to provide early diagnostics and more efficient and frequently personalized therapies for many severe diseases. Nanomaterials enhance the possibilities of medical therapy and diagnostics in many aspects: i) nanomaterials can be used to image specific regions via their fluorescence or plasmonic properties so they can be applied in the analysis of diseases directly inside the human body. II) nanomaterials can be used as efficient active drug delivery systems. In many cases, it is possible to apply the nanomaterials as theranostic agents allowing to monitor the effect of a drug in vivo.

It is commonly recognized that there is no single nanoplatform that can be used in a diagnosis and treatment of more kinds of diseases. Each and every nanoplatform must be precisely tuned to maximise its in vivo effectiveness, while maintaining low toxicity for normal cells and tissues. It is also well known that the biological effects of nanomaterials considerably vary even with superficial changes in its morphology or size. Therefore, many efforts have been given to the development of precision synthesis protocols. Moreover, advanced functionalization methods allow the possibility of incorporating an increasing number of functionalities, which further allow to detect various biological responses, which further allow to target specific cells or sub-cellular compartments, or which can be used in an imaging of drug release.

At our research group, we tackle these scientific challenges with the primary goal to design efficient nanoplatforms and establish new protocols to address severe diseases, including osteosarcoma, breast adenosarcoma or glioblastoma.

Synthesis and functionalization of advanced nanostructured materials for active drug delivery systems, plasmonic or fluorescent based detection of clinically relevant molecular markers and chiral analysis.

  1. Development of drug delivery systems based on i) advanced 2D nanomaterials and ii) biopolymeric nanocarriers.
  2. Development of methods for a detection of clinically relevant molecular markers using optical methods and plasmonic nanostructures.
  3. Analysis of chiral compounds using 2D optical anisotropic nanomaterials.
RANC, V., Z. MARKOVÁ, M. HAJDÚCH, R. PRUCEK, L. KVÍTEK, J. KAŠLÍK, K. ŠAFÁŘOVÁ a R. ZBOŘIL. Magnetically Assisted Surface-Enhanced Raman Scattering Selective Determination of Dopamine in an Artificial Cerebrospinal Fluid and a Mouse Striatum Using Fe3O4/Ag Nanocomposite. Analytical Chemistry. 2014, 86(6), 2939-2946, ISSN 0003-2700, IF: 5.636, PMID: 24555681
CHALOUPKOVA, Z., A. BALZEROVA, Z. MEDRIKOVA, J. SROVNAL, M. HAJDÚCH, K. CEPE, V. RANC a R. ZBOŘIL. Label-free determination and multiplex analysis of DNA and RNA in tumor tissues. Applied Materials Today. 2018, 12(2018), 85-91, ISSN 2352-9407, IF: 1.691,

Impact Factor Journals

BORKOVÁ, L., I. FRYDRYCH, B. VRÁNOVÁ, N. JAKUBCOVÁ, B. LIŠKOVÁ, P. DŽUBÁK, P. PAVLIŠ, M. HAJDÚCH a M. URBAN. Lupane derivatives containing various aryl substituents in the position 3 have selective cytostatic effect in leukemic cancer cells including resistant phenotypes. European Journal of Medicinal Chemistry. 2022, 244, 114850, ISSN 0223-5234, IF: 7.088, PMID: 36283179
HODOŇ, J., I. FRYDRYCH, Z. TRHLÍKOVÁ, J. POKORNÝ, L. BORKOVÁ, S. BENICKÁ, M. VLK, B. LIŠKOVÁ, A. KUBÍČKOVÁ, M. MEDVEDÍKOVÁ, M. PISÁR, J. ŠAREK, V. DAS, A. LIGASOVÁ, K. KOBERNA, P. DŽUBÁK, M. HAJDÚCH a M. URBAN. Triterpenoid pyrazines and pyridines - Synthesis, cytotoxicity, mechanism of action, preparation of prodrugs. European Journal of Medicinal Chemistry. 2022, 243, 114777, ISSN 0223-5234, IF: 7.088, PMID: 36174412
GIUSTO, E., L. ŽÁRSKÁ, D. BEIRNE, A. ROSSI, G. BASSI, A. RUFFINI, M. MONTESI, D. MONTAGNER, V. RANC a G. PANSERI. Graphene Oxide Nanoplatforms to Enhance Cisplatin-Based Drug Delivery in Anticancer Therapy. Nanomaterials. 2022, 12(14), 2372, ISSN 2079-4991, IF: 5.719, PMID: 35889596
KUBÍČKOVÁ, A., Z. MACEČKOVÁ, P. VOJTA, M. ONDRA, J. VOLEJNÍKOVÁ, P. KOŘALKOVÁ, A. JUNGOVA, O. JAHODA, R. MOJZIKOVA, I. HADAČOVÁ, J. CERMAK, M. HORVÁTHOVÁ, D. POSPÍŠILOVÁ a M. HAJDÚCH. Missense mutation in RPS7 causes Diamond-Blackfan anemia via alteration of erythrocyte metabolism, protein translation and induction of ribosomal stress. Blood Cells, Molecules & Diseases. 2022, 97, 102690, ISSN 1079-9796, IF: 2.372, PMID: 35871033
POKORNÝ, J., D. OLEJNÍKOVÁ, I. FRYDRYCH, B. LIŠKOVÁ, S. GURSKÁ, S. BENICKÁ, J. ŠAREK, J. KOTULOVÁ, M. HAJDÚCH, P. DŽUBÁK a M. URBAN. Substituted dienes prepared from betulinic acid - Synthesis, cytotoxicity, mechanism of action, and pharmacological parameters. European Journal of Medicinal Chemistry. 2021, 224, 113706, ISSN 0223-5234, IF: 6.514, PMID: 34311159
MAKOVA, B., V. MIK, B. LIŠKOVÁ, G. GONZALES, D. VÍTEK, M. MEDVEDÍKOVÁ, B. MONFORT, V. RUČILOVÁ, A. KADLECOVA, P. KHIRSARIYA, Z. BARREIRO, L. HAVLICEK, M. ZATLOUKAL, M. SOURAL, K. PARUCH, B. D´AUTREAUX, M. HAJDÚCH, M. STRNAD a J. VOLLER. Cytoprotective activities of kinetin purine isosteres. Bioorganic & Medicinal Chemistry. 2021, 1(33), 115993, ISSN 0968-0896, IF: 3.073, PMID: 33497938
SVEC, P., Z. NOVÝ, J. KUCKA, M. PETŘÍK, O. SEDLACEK, M. KUCHAR, B. LIŠKOVÁ, M. MEDVEDÍKOVÁ, K. KOLOUCHOVA, O. GROBORZ, L. LOUKOTOVA, R. KONEFAL, M. HAJDÚCH a M. HRUBY. Iodinated Choline Transport-Targeted Tracers. Journal of Medicinal Chemistry. 2020, 63(24), 15960–15978, ISSN 0022-2623, IF: 6.205, PMID: 33271015
KARASKOVA, E., D. POSPÍŠILOVÁ, M. VELGANOVA-VEGHOVA, M. GERYK, J. VOLEJNÍKOVÁ, D. HOLUB a M. HAJDÚCH. Importance of Hepcidin in the Etiopathogenesis of Anemia in Inflammatory Bowel Disease. Digestive Diseases and Sciences. 2020, 66(10), 3263-3269, ISSN 0163-2116, IF: 2.751, PMID: 33063192
SMOLKA, V., M. ROHANOVA, B. LUDÍKOVÁ, Z. NOVÁK, M. ZAPALKA, D. POSPÍŠILOVÁ a J. VOLEJNÍKOVÁ. Severe linezolid-induced lactic acidosis in a child with acute lymphoblastic leukemia: A case report. Journal of Infection and Chemotherapy. 2020, 26(12), 1316-1318, ISSN 1341-321X, IF: 1.722, PMID: 32859497
DVOŘANOVÁ ŠTĚPÁNKOVÁ, J., M. KUGLER, J. HOLUB, V. SICHA, V. DAS, J. NEKVINDA, S. EL ANWAR, M. HAVRANEK, K.. POSPISILOVA, M. FABRY, V. KRAL, M. MEDVEDÍKOVÁ, S. MATEJKOVA, B. LIŠKOVÁ, S. GURSKÁ, P. DŽUBÁK, J. BRYNDA, M. HAJDÚCH, B. GRUNER a P. ŘEZÁČOVÁ. Sulfonamido Carboranes as Highly Selective Inhibitors of Cancer-Specific Carbonic Anhydrase IX. European Journal of Medicinal Chemistry. 2020, 200, 112460, ISSN 0223-5234, IF: 5.572, PMID: 32505851
VESELOVSKA, L., N. KUDLOVÁ, S. GURSKÁ, B. LIŠKOVÁ, M. MEDVEDÍKOVÁ, O. HODEK, E. TLOUŠŤOVÁ, N. MILISAVLJEVIC, M. TICHÝ, P. PERLÍKOVÁ, H. MERTLIKOVA-KAISEROVA, J. TRYLCOVA, R. POHL, B. KLEPETAROVA, P. DŽUBÁK, M. HAJDÚCH a M. HOCEK. Synthesis and Cytotoxic and Antiviral Activity Profiling of All-Four Isomeric Series of Pyrido-Fused 7-Deazapurine Ribonucleosides. Chemistry. 2020, 26(57), 13002-13015, ISSN 0947-6539, IF: 4.857, PMID: 32275109
VOLEJNÍKOVÁ, J., P. VOJTA, H. URBANKOVA, R. MOJZIKOVA, M. HORVÁTHOVÁ, I. HOCHOVA, J. CERMAK, J. BLATNY, M. SUKOVA, E. BUBANSKA, J. FEKETOVA, D. PROCHAZKOVA, J. HORAKOVA, M. HAJDÚCH a D. POSPÍŠILOVÁ. Czech and Slovak Diamond-Blackfan Anemia (DBA) Registry update: Clinical data and novel causative genetic lesions. Blood Cells, Molecules & Diseases. 2020, 81, 102380, ISSN 1079-9796 , IF: 2.305, PMID: 31855845
BORKOVÁ, L., I. FRYDRYCH, N. JAKUBCOVÁ, R. ADÁMEK, B. LIŠKOVÁ, S. GURSKÁ, M. MEDVEDÍKOVÁ, M. HAJDÚCH a M. URBAN. Synthesis and biological evaluation of triterpenoid thiazoles derived from betulonic acid, dihydrobetulonic acid, and ursonic acid. European Journal of Medicinal Chemistry. 2019, 185, 111806, ISSN 0223-5234, IF: 4.833, PMID: 31677446
GRUNER, B., J. BRYNDA, V. DAS, V. SICHA, J. ŠTĚPÁNKOVÁ, J. NEKVINDA, J. HOLUB, K.. POSPISILOVA, M. FABRY, P. PACHL, V. KRAL, M. KUGLER, V. MAŠEK, M. MEDVEDÍKOVÁ, S. MATEJKOVA, A. NOVÁ, B. LIŠKOVÁ, S. GURSKÁ, P. DŽUBÁK, M. HAJDÚCH a P. ŘEZÁČOVÁ. Metallacarborane Sulfamides: Unconventional, Specific, and Highly Selective Inhibitors of Carbonic Anhydrase IX. Journal of Medicinal Chemistry. 2019, 62(21), 9560-9575, ISSN 0022-2623, IF: 6.054, PMID: 31568723
KARASKOVA, E., J. VOLEJNÍKOVÁ, D. HOLUB, M. VELGANOVA-VEGHOVA, M. SPENEROVA, V. MIHÁL a D. POSPÍŠILOVÁ. Changes in serum hepcidin levels in children with inflammatory bowel disease during anti-inflammatory treatment. Journal of Paediatrics and Child Health. 2019, 56(2), 276-282, ISSN 1034-4810, IF: 1.688, PMID: 31411363
TOKARENKO, A., B. LIŠKOVÁ, S. SMOLEN, N. TÁBORSKÁ, M. TICHÝ, S. GURSKÁ, P. PERLÍKOVÁ, I. FRYDRYCH, E. TLOUŠŤOVÁ, P. ZNOJEK, H. MERTLIKOVA-KAISEROVA, L. POSTOVA SLAVETINSKA, R. POHL, B. KLEPETAROVA, N. KHALID, Y. WENREN, R. LAPOSA, P. DŽUBÁK, M. HAJDÚCH a M. HOCEK. Synthesis and Cytotoxic and Antiviral Profiling of Pyrrolo- and Furo-fused 7-Deazapurine Ribonucleosides. Journal of Medicinal Chemistry. 2018, 61(20), 9347-9359, ISSN 0022-2623, IF: 6.253, PMID: 30281308
KARASKOVA, E., J. VOLEJNÍKOVÁ, D. HOLUB, M. VELGANOVA-VEGHOVA, L. SULOVSKÁ, V. MIHÁL, M. HORVÁTHOVÁ a D. POSPÍŠILOVÁ. Hepcidin in newly diagnosed inflammatory bowel disease in children. Journal of Paediatrics and Child Health. 2018, 54(12), 1362-1367, ISSN 1034-4810, IF: 3.595, PMID: 29923651
MEDKOVA, A., J. SROVNAL, J. POTOMKOVA, J. VOLEJNÍKOVÁ a V. MIHÁL. Multifarious diagnostic possibilities of the S100 protein family: predominantly in pediatrics and neonatology. World Journal of Pediatrics. 2018, 14(4), 315-321, ISSN 1708-8569, IF: 1.164, PMID: 29858979
VOLEJNÍKOVÁ, J., J. ZAPLETALOVÁ, M. JAROSOVA, H. URBANKOVA, E. KLASKOVA, M. HOROWITZ, M. HAJDÚCH a V. MIHÁL. Acute lymphoblastic leukemia in a child with Leri-Weill syndrome and complete SHOX gene deletion: A Case Report. Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic. 2018, 162(1), 65-70, ISSN 1213-8118, IF: 0.894, PMID: 29469136
VOLEJNÍKOVÁ, J., J. HORACEK a F. KOPŘIVA. Dapsone treatment is efficient against persistent cutaneous and gastrointestinal symptoms in children with Henoch-Schönlein purpura. Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic. 2018, 162(2), 154-158, ISSN 1213-8118, IF: 1.087, PMID: 29097818
JOUROVA, L., P. ANZENBACHER, B. LIŠKOVÁ, Z. RÁCOVÁ (MATUŠKOVÁ), P. HERMANOVA, T. HUDCOVIC, H. KOZAKOVA, L. HRNCIROVA a E. ANZENBACHEROVÁ. Colonization by non-pathogenic bacteria alters mRNA expression of cytochromes P450 in originally germ-free mice. Folia Microbiologica. 2017, 62(6), 463-469, ISSN 0015-5632 , IF: 1.335, PMID: 28337589
TICHÝ, M., S. SMOLEN, E. TLOUŠŤOVÁ, R. POHL, T. OŽDIAN, K. HEJTMÁNKOVÁ, B. LIŠKOVÁ, S. GURSKÁ, P. DŽUBÁK, M. HAJDÚCH a M. HOCEK. Synthesis and Cytostatic and Antiviral Profiling of Thieno-Fused 7-Deazapurine Ribonucleosides. Journal of Medicinal Chemistry. 2017, 60(6), 2411-2424, ISSN 0022-2623, IF: 5.589, PMID: 28221790
JAROSOVA, M., J. VOLEJNÍKOVÁ, I. PORIZKOVA, M. HOLZEROVA, D. POSPÍŠILOVÁ, Z. NOVÁK, J. VRBKOVÁ a V. MIHÁL. Chromosomal aberrations in childhood acute lymphoblastic leukemia: 15-year single center experience. Cancer Genetics. 2016, 209(7-8), 340-347, ISSN 2210-7762, IF: 1.930, PMID: 27341996
VOLEJNÍKOVÁ, J., V. BAJCIOVA, L. SULOVSKÁ, M. GEIEROVÁ, E. BURIANKOVA, M. JAROSOVA, M. HAJDÚCH, J. STERBA a V. MIHÁL. Bone marrow metastasis of malignant melanoma in childhood arising within a congenital melanocytic nevus. Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czech Republic. 2016, 160(3), 456-460, ISSN 1213-8118 , IF: 0.894, PMID: 27049531
VOLEJNÍKOVÁ, J., M. JAROSOVA, D. POSPÍŠILOVÁ, Z. NOVÁK, J. VRBKOVÁ, M. HAJDÚCH a V. MIHÁL. Treatment and prognosis of childhood acute lymphoblastic leukemia on protocols ALL-BFM 90, 95 and ALL IC-BFM 2002: a retrospective single-center study from Olomouc, Czech Republic. Neoplasma. 2016, 63(3), 456-61, ISSN 0028-2685, IF: 1.871, PMID: 26925793
SAINI, H., P. KALLEM, E. OTYEPKOVA, F. GEYER, A. SCHNEEMANN, V. RANC, F. BANAT, R. ZBOŘIL, M. OTYEPKA, R. FISCHER a K. JAYARAMULU. Two-dimensional MOF-based liquid marbles: surface energy calculations and efficient oil-water separation using a ZIF-9-III@PVDF membrane. Journal of Material chemistry A. 2021, 9(41), 23651-23659, ISSN 2050-7488, IF: 12.732,

Other Reviewed Journals

GERYK, M., E. KARASKOVA, M. VELGANOVA-VEGHOVA, B. LUDÍKOVÁ, J. CIVRNY a J. VOLEJNÍKOVÁ. Objemný gastroduodenální trichobezoár (Rapunzel syndrom) jako příčina bolestí břicha a nechutenství u devítileté dívky. Česko-slovenská pediatrie 2021. 2021, 76(4), 233-237. ISSN 0069-2328.
Project: Pharmacokinetic methods in preclinical drug testing
Supervisors: Hajdúch Marián M.D., Ph.D., Lišková Barbora Ph.D.
Available: 1
Intended for: Doctoral training

The study of ADME properties of a potential drug that are part of the IMTM chemical library - obtained by national or international cooperation belongs to one of the first steps in predicting potential drugs. In vitro models generate many ADME parameters, including chemical, plasma and microsomal stability, plasma protein binding and proportion of passive diffusion as a transport mechanism. The Caco-2 and MDCK-MDR1 permeability assays are established models of intestinal and blood-brain barriers, respectively. Analysis of samples is performed using a Agilent RapidFire 300 - rapid online solid phase extraction with subsequent detection of the mass spectrometer Qtrap 5500 (AB Sciex) - RF/MS. High-Throughput Mass Spectrometry System RF/MS delivers ultrafast, label – free analysis of native compounds for biochemical assays in ADME and provide throughput speeds of 6 to 10 seconds per sample.

Group Leader

Ranc Václav Ph.D.

Group members

Lišková Barbora Ph.D.


Volejníková Jana Ph.D.

Postdoctoral Fellow, Staff