Graduated doctoral students
Khushboo Agrawal
Status: Graduated
Specialization:
5-Azacytidine Nucleosides and their Derivatives: Molecular Hallmarks of Drug Resistance & Alternative Therapeutic Regimen
The thesis is mainly focused towards the investigation of the mechanisms of chemotherapeutic resistance to DNA methylation inhibitors. The study will aid to the understanding of the molecular basis of acquired tumor resistance and help in predicting its clinical response, as well as in designing alternative treatment regimens for overcoming resistance.Narendran Annadurai
Status: Graduated
Specialization:
Identification and Characterisation of Tau Minimal Regions as Potential Pharmacological Targets Against Tau Spreading in Tauopathies
Understanding the mechanisms of tau aggregation, propagation and cellular machinery involved in aggregated tau degradation would help identify therapeutic targets for tauopathies (Alzheimer’s disease, PSP, CTE, FTLD, etc.) and other neurodegenerative diseases. The thesis is focused on studying tau aggregate spreading mechanisms and identifying small molecule inhibitors of tau misfolding and aggregation using in vitro aggregation assays, cell and molecular biology, biochemistry and microscopy techniques. I am also working on validating small molecules (selected from in vitro MARK4 kinase activity inhibition assay) on inhibiting microtubule affinity regulating kinase 4 (MARK4) mediated tau phosphorylation using a cell line model.Lucie Borková
Status: Graduated
Specialization:
Modification of triterpenes with the use of transition metal catalyzed reactions and nucleophilic substitution
In this work, a larger study of transition metal catalyzed reaaction of triterpenes should be studied in order to find a new approach for a preparation of novel derivatives with high cytotoxic activity and favorable pharmacological properties.Radana Brumarová
Status: Graduated
Specialization:
Study of patobiochemistry of inherited metabolic disorders by metabolomic approach
The thesis is focused on the targeted and untargeted metabolomic analysis of selected diseases by liquid chromatography coupled with tandem mass spectrometry and high resolution mass spectrometry. The main goal is the identification of biomarkers and understanding changes in metablolic pathways important for patobiochemistry of inherited disorders.Alžběta Gardlo
Status: Graduated
Specialization:
Multidimensional statistical methods for analysis of human metabolome
The aim of the thesis is to study the possibility of using advanced multivariate statistical methods for statistical analysis of metabolomical data, including the use of appropriate software tools. The main part of the thesis is the application of the methodology of logratio analysis of compositional data.Dušan Holub
Status: Graduated
Specialization:
Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LCMS
Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. As such, it has potential as an indicator of any iron-related condition, such as anemia. My research focuses on the measurement of hepcidin in biological fluids to improve our understanding of iron-related disorders. A possible result of such studies could be the development of hepcidin as a tool for diagnosis and clinical management of such disorders.Barbora Hošíková
Status: Graduated
Specialization:
In vitro study of the effect of the photosensitive substance on the tumor cell lines
This study deals with utilization of newly synthetised photosensitizers chemically clasified as phthalocyanines in photodynamic therapy.The photosensitizers are tested in vitro on tumor cell line – HeLa (cervical cancer cells) and non-tumor cell line NIH3T3 (mouse fibroblasts). A main goal is to find the photosensitizer causing the highest phototoxic effect on the tumor cells with minimal side effects.Magdalena Houdová Megová
Status: Graduated
Specialization:
Cytogenetics changes in solid tumors
The thesis is focused on the identification of molecular and genetic changes in solid tumors focusing on glial tumors especially on a group of recurrent glioblastoma. The main goal is to identify molecular and genetic aberrations harboured by glial tumors and selected these which are responsible for glial tumor progression and recurrence.Miroslava Htoutou Sedlakova
Status: Graduated
Specialization:
Clinical impact of extended-spectrum beta-lactamases
The most frequent mechanism of resistance to beta-lactam antibiotics in Gram-negative bacteria is production of beta-lactamases. Those having serious clinical significance are mainly ESBL and AmpC broad-spectrum beta-lactamases and, recently, carbapenemases. For adequate antibiotic therapy of infections caused by producers of these enzymes, it is necessary to not only rapidly and correctly identify the etiological agents and their susceptibility to antibiotics but also detect the presence of these dangerous phenotypes of resistance. One of the objectives of this dissertation was to determine the sensitivity of phenotypic methods for detecting broad-spectrum beta-lactamases and carbapenemases and to select the most suitable ones for routine microbiology practice.Sylwia Jančík
Status: Graduated
Specialization: Pediatrics
New strategies for genotyping of KRAs and BRAF onkomarkesrs in heterogeneous clinical tumors samples
The thesis is focused on genotyping of KRAS and BRAF in heterogeneous clinical tumor samples using different molecular genetics methods (Sanger direct sequencing, COLD-PCR, CE-IVD PCR Real-Time methods, and massively parallel sequencing).Hana Jaworek
Status: Graduated
Specialization:
Human papillomavirus and its significance for diagnostics and therapeutics
The thesis is focused on influence of HPV infection on fertility and cancer development. HPV DNA is detected by PCR based diagnostics and in situ hybridisation. The main goal is to determine an influence of HPV on fertility and cancer development.Petr Konečný
Status: Graduated
Specialization:
Screening of chemical compounds´ biological activities in vitro
The thesis is focused on the identification of potentially active anticancer, antibacterial and antiparasitic compounds. Using flow cytometric evaluation provides information about cell cycle modifications after treatment with different chemical compounds and allows selection of most potent agents for further testing or synthesis of more efficient analogues.Jana Kotulová
Status: Graduated
Specialization:
Identification of adenosine receptor modulators and their anticancer properties
Adenosine receptors are involved in several cellular processes and can play significant role in development of various diseases, including cancer. The aim of the thesis is to study the role of adenosine receptors in cell physiology as well as in pathological state. This work is focused on screening of potential ligands of adenosine receptors and characterization of their molecular interactions using high-throughput screening approach and further validation by methods of molecular biology. Novel potent inhibitors can be beneficial for cancer therapy in the future.Soňa Krajčovičová
Status: Graduated
Specialization:
Solid-phase synthesis of heterocyclic compounds and study of their biological properties
This work will be focused on development of synthetic pathways applicable for high-throughput synthesis of organic compounds containing heterocyclic moiety and further evaluation of their biological properties.Petra Králová
Status: Graduated
Specialization:
Diversity-Oriented Synthesis Using Immobilized 2/4-Nitrobenzensulfonamides As The Key Intermediates
The Ph.D. thesis is focused on the diversity-oriented synthesis using immobilized 2/4-nitrobenzensulfonamides as the key intermediates to prepare single and fused nitrogenous heterocycles. The preparation of these derivatives was performed using solid-phase synthesis (SPS) that yielded the common intermediate, i.e. α-amino ketone which was further modified to final products. The general concept of SPS and its (dis)advantages are briefly discussed in Introduction of this thesis. The following subchapter introduces the previous research in the field of N-sulfonyl and N-acyl morpholine-3-carboxylic acid derivatives which has become a major inspiration for all of our projects described in this thesis. The following chapter Aims of the work provides the specific goals in more details. After that, the thesis continues as the State of the art to display shortly the general application of immobilized 2/4-nitrobenzensulfonamides and α-amino ketones to prepare diverse heterocycles. This subchapter is supplemented by our review article summarizing the polymer-supported syntheses of heterocycles bearing oxazine and thiazine scaffolds which is attached in Appendix B at the end of this thesis. Then the part of Results and discussion follows which is divided into eight subchapters according to the individual synthetic projects. In Conclusion part, all results are briefly summarized.Natálie Kudlová
Status: Graduated
Specialization:
High throughput sequencing methods in biomarker identification and validation
High throughput sequencing is rapidly improving in its quality, speed and cost. This scientific approach increases our ability to characterise diseases at the genomic, transcriptomic and epigenetic levels. The aim of the thesis is to use this technique for studying cells under ribosomal stress. The work is focused on the identification and validation of biomarkers that are in connection with ribosomal stress caused diseases.Zuzana Macečková
Status: Graduated
Specialization:
Molecular pathology of Diamond-Blackfan anemia
Diamon-Blacfan anemia is a rare congenital red cell aplasia, often associated with congenital malformations, particularly of upper limbs and craniofacial region. As a cause, mutations in genes for ribosomal proteins have been reported in approximately 50% of the cases. Mutations in ribosomal proteins lead to defects during ribosome biogenesis and thus ribosome stress, which in turn leads to cell cycle arrest or apoptosis in erythroid progenitors.In my study, I focus on discovering and validation of new possible molecular causes of Diamond-Blackfan anemia and other ribosomopathies by NGS approach followed by functional studies of chosen genes.Jakub Malohlava
Status: Graduated
Specialization:
Study of photodynamically active substances using modern microscopic techniques
The thesis is focused on the evaluation of substances used for inducing photodynamic effect in cancerous cells. The main aim is to assess the effect on mechanical properties using atomic force microscopy.Mariia Matveieva
Status: Graduated
Specialization:
Automatic mining of relationships between structure and activity in chemical databases
The thesis is focused on the development of methods and tools for mining valuable structural features of compounds in chemical databases. Those methods are being developed with the main goal to explain observed structure-activity relationships and to design new molecules with improved properties.Kateřina Mičová
Status: Graduated
Specialization:
Significance of intracellular and plasma levels of new tyrosine kinase inhibitors
The thesis is focused on development of HPLC-MS/MS methods for determination of tyrosine kinase inhibitors imatinib, nilotinib and dasatinib used for the treatment of chronic myeloid leukemia, and also on relationship between imatinib plasma levels and treatment response.Lucie Navrátilová
Status: Graduated
Specialization:
Identification and typing of nonfermenting gram-negative rods recovered from lungs of cystic fibrosis and chronic osbstructive pulmonary disease (COPD) patients
The thesis is focused on the developent of novel approaches to identification and typing of bacterial pathogens combining the technique of PCR and high-resolution melting analysis (HRMA) with the aim to translate the advances of economic and rapid molecular techniques into routine diagnostics in favor of best tailored antimicrobial therapy in chronic lung diseases.Tomáš Oždian
Status: Graduated
Specialization:
Cancer proteomics in clinical and experimental oncology
The thesis is focused on the detection possible biomarkers of cancer treatment efficiancy. Main task is to prepare database of proteomic profiles of current clinically available anticancer drugs.Klára Pížová
Status: Graduated
Specialization:
Effect of electromagnetic radiation and ultrasound on cell lines
The thesis is focused on in vitro testing of different sensitizers for photodynamic and sonodynamic treatment. The main goal is to identify the best sensitizer concentrations and total radiation doses and identify mechanisms of their action.Jan Pokorný
Status: Graduated
Specialization:
Modifications of double bonds in triterpenes with antitumor activity
In this work, double bond of betulin, betulinic acid and other cytotoxic triterpenes will be modified by various methods. First method will be based on transition metal catalyzed crosscoupling reactions, of triterpenic boronic acids with a variety of other molecules. Second, aldehyde will be prepared in the neighborhood of the double bond and it will be condensed with a number of organic hydrazines, hydrazones, amines etc.Zuzana Rácová (Matušková)
Status: Graduated
Specialization:
Cytochromes P450 - main enzymes in drug metabolism: Study of properties of these enzymes in rat gastrointestinal tract and their influence by probiotics
The study is focused on effect of probiotics on cytochromes P450 and pharmacokinetics of concomitatntly taken drug.Veronika Ručilová
Status: Graduated
Specialization:
Development of Strategies for Solid-Phase Synthesis of Nitrogenous Heterocyclic Compounds Based on the 2- and 4-Nitrobenzenesulfonamide Chemistry
The submitted doctoral thesis deals with the development of simple procedures applicable for the preparation of libraries of selected heterocyclic compounds. In all of the introduced procedures, the immobilized 2- or 4-nitrobenzenesulfonamides were used as the key intermediates. Different scenarios have been used in which the nitrobenzenesulfonyl (Nos) group was applied (1) only as a protective/activating group for Fukuyama selective monoalkylation followed by the cleavage of the sulfonamide scaffold, (2) as a building block after previous Fukuyma alkylation, or (3) as a building block without application of Fukuyama alkylation. The key intermediates were further modified and converted to the target products under different cyclization conditions. During our long-term research we have developed high-throughput solid-phase syntheses of various heterocyclic compounds involving six types of different scaffolds.Gabriela Rylová
Status: Graduated
Specialization:
Identification of molecular targets of anticancer drugs by proteomic methods
The thesis is focused on the identification of molecular targets and mechanism of action of quinolone derivatives with anticancer properties. The method used for this purpose is affinity purification coupled to mass spectrometry. Obtained data will be used for structure optimization of these potential anticancer compounds.Jiří Řehulka
Status: Graduated
Specialization:
Mitochondria as a therapeutic target in cancer
Mitochondria are organelles involved in cellular energy metabolism. This aspect of the cell-physiology is often altered during neoplastic transformation/carcinogenesis. For this reason, mitochondria constitute potential targets in cancer therapy. My work focuses on the anti-cancer activity of novel derivatives of betulinic acid in the context of drug-discovery.Anna Sťahelová
Status: Graduated
Specialization:
Genetic factors in complex diseases with inflammatory compound in pathogenesis
The thesis is focused on the genetic association studies (GAS) in complex diseases. GAS use the comparison of genetic markers (Single Nucleotide Polymorphisms or Copy Number Variations) between the patients with complex diseases and healthy controls. The main goal is to detect susceptibility genes responsible for heritable component of complex diseases.Dana Šimková
Status: Graduated
Specialization:
Study of invasive growth and its blocation possibilities in breast and prostate cancer
Thesis is focused on an identification of asporin‘s (protein interacting with extracellular matrix) role in motility and invasiveness of model cell lines. The main goal is to determine if asporin could be considered as a marker of tumor metastatic behaviour.Zdeněk Škrott
Status: Graduated
Specialization:
The role of the ubiquitin-proteasome system in tumorigenesis and cancer treatment
Regulated protein degradation is mediated by ubiquitin-proteasome system. Inhibition of proteasome proteolytic activity is a promising approach in cancer therapy with first-in-class drug bortezomib approved for the treatment of patients with multiple myeloma. Since ubiquitination regulates not only protein degradation but also wide variety of cellular signaling pathways, this new approach might bring new drugs with better efficacy and safety profiles.Zuzana Šporiková
Status: Graduated
Specialization:
Cytogenetics changes in solid tumors
The thesis is focused on the identification of molecular and genetic changes in solid tumors focusing on glial tumors especially on a group of recurrent glioblastoma. The main goal is to identify molecular and genetic aberrations which are responsible for glioblastoma progression and recurrence by comprehensive, complementary molecular genetic methods and high-throughput technologies.Michaela Šváchová
Status: Graduated
Specialization:
Importance of molecular markers for cancer diagnostics
The thesis is focused on the detection of cancer associated markers. Emphasis is on importance of polymerase chain reaction based gene rearrangements in the diagnosis of lymphoproliferative disorders in biopsy samples. These results have confirmed that the PCR-based analysis is suitable as a routine method and is helpful in establishing a diagnosis in morphologically unclear cases.Jana Václavková
Status: Graduated
Specialization:
Identification of mechanisms of action of molecules with anticancer effect
The thesis is focused on optimization of the method for identifying cellular targets of anticancer drugs. The aim of the work is to optimize affinity purification using streptavidin coated plates. Next the new drugs will be tested to find their molecular target and the validation studies will be performed.Renata Večeřová
Status: Graduated
Specialization:
Možnosti testování antimikrobní aktivity stávajících a nově vyvíjených látek
Cílem práce je definovat antimikrobní účinky nových látek, především nanočástic stříbra. Bude testován účinek na standardní referenční bakteriální kmeny, multirezistentní bakterie a na vybrané kvasinky. Na základě výsledků budou analyzovány potencionální praktické možnosti těchto látek.Ivo Vrobel
Status: Graduated
Specialization: