Current doctoral students
Jan Bachořík
Status: Ongoing
Specialization:
Preparation of semisynthetic triterpenes -
Zuzana Barbuščáková
Status: Ongoing
Specialization:
Molecular system for multiple detection of enzymes inside the cell -
Kateřina Bendová
Status: Ongoing
Specialization:
Multimodal imaging for in vivo testing of selected biomolecules -
Ondřej Bouška
Status: Ongoing
Specialization:
Pathological conditions associated with human papillomavirus infection -
Dominika Flašarová
Status: Ongoing
Specialization:
Effect and toxicity of HIPEC -
Jaroslava Friedecká
Status: Ongoing
Specialization:
Metabolomic GC×GC-TOF MS analysis of biofluids The thesis is focused on metabolomic analysis of biofluids, using comprehesnive two-dimensional gas chromatography coupled with time of flight mass spectrometry (GC×GC-TOF MS). The optimal column combination suitable for given biofluid has been evaluated, GC×GC TOF-MS analysis has been optimized, separation efficiency has been determined, data processing method has been developed.
Mária Gamratová
Status: Ongoing
Specialization: Pathological anatomy and forensic medicine
The role of cancer microenvironment in prognosis of Hodgkin lymfomas -
Veronika Grešáková
Status: Ongoing
Specialization:
Functional characterization of FAM208a -
Anahit Hakobjanyan
Status: Ongoing
Specialization:
Expression and regulation of cytokines or signaling molecules at the interface of bone and joint replacement: a contribution to the immunopathogenesis osteolysis/ periprosthetic infection -
Jiří Hodoň
Status: Ongoing
Specialization:
Cytotoxic active triterpenes and their mechanism of action -
Denisa Hrčková Drozdková
Status: Ongoing
Specialization: Medical biology
Effect of histone deacetylase inhibitors on apoptosis and proliferative activity of cancer cells -
Lenka Hrubá
Status: Ongoing
Specialization:
Mechanism of multidrug resistance (MDR) in cancer cells Drug resistance is one of the major challenges cancer therapy is facing to. Every year, cancer affects millions of people around the world. More than 19 million new cases were diagnosed worldwide in 2020. According to the National Cancer Registry (NOR), there were 67,000 new cases in the Czech Republic, and there were 27,468 deaths associated with cancer diagnosis. More than 10 million patients died worldwide. There can be many reasons for the treatment failure- from late diagnosis of the disease, to limited surgical or radiotherapy options, to drug resistance that greatly reduce the choice of suitable chemotherapeutics. In order to simulate the situation that takes place in the body of a patient treated with substances with cytostatic or cytotoxic effect, it is necessary to choose the right biological model. One of the suitable models seems to be tumor cell lines, which can be used for selection of clones with a resistant phenotype. These cells can be then tested in in vitro and in vivo conditions. In resistant cell lines it is possible to describe the mechanism of drug resistance and open the way for new potential therapies, to verify the sensitivity of resistant tumors to newly developed cytostatics, or to predict how easily and whether resistance to a particular drug will arise at all.
Zlata Hušková
Status: Ongoing
Specialization:
Molecular mechanisms of action of plant steroids on tumor cells -
Alžběta Chabroňová
Status: Ongoing
Specialization: Physiology and pathophysiology
Exosomal miRNAs in chronic obstructive lung disease This study will be focused on identifying mechanisms of post-transcriptional regulation in COPD pathogenesis by measuring expression of selected miRNAs and determining differentially expressed miRNAs in patients vs controls.
Aleksandra Ivanova (Nikonenko)
Status: Ongoing
Specialization:
In silico design of compounds with desired properties -
Eliška Ivanovová
Status: Ongoing
Specialization:
Development and validation of LC/MS methods for diagnostics of inherited metabolic disorders -
Kateřina Ječmeňová
Status: Ongoing
Specialization:
Drug resistance mechanisms in cancer -
Jana Jiravová
Status: Ongoing
Specialization:
The study of nanomaterials using modern microscopic techniques The thesis is focused on the characterisation of nanoparticles properties and detection effect of nanoparticles on biological systems included tumor and non-tumor cell lines. For detection of nanoparticles are used modern microscopic techniques (e.g. atomic force microscopy, Raman spectroscopy, confocal and electron microscopy). Nanoparticles are massively used in daily life, so it is important to know their properties.
Jana Juráňová
Status: Ongoing
Specialization:
The mechanism of wound healing -
Ondřej Jurčeka
Status: Ongoing
Specialization:
Modification of lupine triterpenes in position C-17 -
Barbora Kalousová
Status: Ongoing
Specialization:
Genetic biomarkers of tumors and aging Clonal hematopoiesis of indeterminate potential (CHIP) has recently been described as an age-related condition manifested by the accumulation of somatic mutations in cells of the hematopoietic system. Although this condition is not accompanied by any morphological features of hematological neoplasia, the incidence of CHIP correlates with increased overall mortality and the risk of developing malignant transformation of hematopoietic cells, as well as cardiovascular disease. The investigation of possible links between CHIP, aging, and ischemic stroke is the main aim of this study. We will use various techniques, including FACS, MACS, and advanced deep sequencing allowing highly sensitive detection of mutations in samples of stroke patients. The analysis of immune cells in the blood and also cells in atherosclerotic plaques will be of special interest.
Lenka Kocourková
Status: Ongoing
Specialization:
Effect of coregulators on the androgen receptor activity in cells derived from prostate cancer after their treatment with histone deacetylase inhibitor The thesis is focused on the detection of coregulator expressions in cancer and normal prostate cells. Coregulators, coactivators as well as coregulators, play an important role in androgen receptor-mediated progression of the prostate cancer. There is a competition between coactivators and corepressors for binding on the androgen receptor and therefore, the changes and the expression ratio between could be important for prostate cancer growth and progression. We focus on two regulators: SMRT (silencing mediator for retinoid and thyroid hormone receptors) as corepressor, and p300 as coactivator.
Markéta Kolečková
Status: Ongoing
Specialization:
New diagnostic biomarkers and tumor progression -
Lucie Kotková
Status: Ongoing
Specialization:
Genetic and Epigenetic Biomarkers in cancer -
Štěpán Kouřil
Status: Ongoing
Specialization: Medical chemistry and clinical biochemistry
Lipidomic analysis of biological samples by HR-MS -
Leona Krátká
Status: Ongoing
Specialization:
Stereoselective synthesis on solid support -
Patricia Kriegelsteinová
Status: Ongoing
Specialization:
Synthesis of benzothiadiazepines using solid-phase synthesis -
Agáta Kubíčková
Status: Ongoing
Specialization:
Cellular models for optical screening of biologically active compounds -
Alina Kutlushina
Status: Ongoing
Specialization:
Development of 3D pharmacophore signatures and their application in drug design -
Aleš Kvasnička
Status: Ongoing
Specialization:
Lipidomic analysis of dry blood spots -
Radmila Lemstrová
Status: Ongoing
Specialization:
Prognostic and predictive factors for pancreatic cancer -
Martin Loffelmann
Status: Ongoing
Specialization:
DNA Damage Signalling in the Response to Cell Stress -
Dávid Lukáč
Status: Ongoing
Specialization:
DNA replication and cancer -
Petra Lužná
Status: Ongoing
Specialization:
Methylation status in patients with multiple myeloma -
Lucie Mádrová
Status: Ongoing
Specialization:
Study of the effect of selected cancerostatics on the cell metabolome The thesis is focused on the development of both targeted and untargeted metabolomics methods (liquid chromatography coupled with high resolution mass spectrometry) for the study of the effects of various anticancer drugs on model cell lines. The main goal is to quantitatively analyse changes in metabolome’s composition for the comprehensive characterization of metabolic networks.
Zuzana Machačová
Status: Ongoing
Specialization:
Molecular mechanisms regulating DNA replication -
Zuzana Malá
Status: Ongoing
Specialization:
Nanomaterials and the study of cell damage fluorescence methods The thesis is focused on the study of effect of various porphyrin and phtalocyanin photosensitizers during different light intensity on cell and bacterial lines using fluorescent, molecular and microscopis methods. Further the thesis is focused on the characterisation of nanoparticles properties combinated with photodynamic therapy.
Lukáš Malina
Status: Ongoing
Specialization:
Study of cell destruction using physical and chemical methods The thesis is focused on the study of effect of various porphyrin and phtalocyanin photosensitizers during different light intensity on G361 (human skin melanoma) and NIH3T3 (mouse fibroblasts) cell lines using fluorescent, molecular and microscopic methods. Main aim is on the dependence of photodynamic therapy on photosensitizer type, its concentration and light doses on tumorous and non-tumorous lines to choose best fitting photosensitizer.
Martina Matějková
Status: Ongoing
Specialization: Oncology
Expression profiling effects of anticancer drugs -
Marcel Matzenauer
Status: Ongoing
Specialization:
Evaluation of the effectiveness and toxicity of stereotactic radiotherapy -
Martina Medvedíková
Status: Ongoing
Specialization:
Study of interaction of biologically active small molecules with selected target macromolecules The thesis is focused on the study of the interaction potential anticancer drugs with target macromolecules (DNA, proteins). These interactions are analyzed using various methods, such as isothermal titration calorimetry, confocal Raman microscopy and molecular biology methods.
Alena Mičková
Status: Ongoing
Specialization:
Monitoring characteristics of tumor stem cells from prostate cancer -
Jaroslav Michálek
Status: Ongoing
Specialization:
Molecular aspects of atherosclerosis (focusing on microRNA) -
Guzel Minibaeva
Status: Ongoing
Specialization:
De novo design of syntheticaly feasible molecules -
Eva Novosadová
Status: Ongoing
Specialization:
Role of RBPs (RNA- binding proteins) in regulation of immune reactions In this thesis will be studied RNA- binding proteins on their expression level. Further will be investigated their possible role of biomarkers in patients with inflammatory lung diseases.
Martin Ondra
Status: Ongoing
Specialization:
Development and validation of luminescent HTS tool for discovery and optimization of novel combination of CFTR correctors and modifiers Cystic fibrosis (CF) disease results from mutations in the CFTR gene that encodes for an epithelial membrane protein acting as a chloride ion channel and water transport regulator. The mutations affecting CFTR protein expression result in difficulties in processing, folding, function and/or trafficking to the membrane depending on the type of mutations. Future discoveries of CFTR modulators and correctors rely on screening many chemical libraries. To develop such a screening tool suitable for high-throughput screening (HTS), a cellular model based on the combination of CRISPR/Cas9 technology and HiBiT tag will be prepared.
Zuzana Pastorková
Status: Ongoing
Specialization:
Influence of microRNA to metastasis -
Martin Pola
Status: Ongoing
Specialization:
The role of oxygen in the effectiveness of photodynamic therapy on cellular models in the presence of sensitizers -
Julie Rendlová
Status: Ongoing
Specialization: Applied mathematics
Advanced methods of compositional data analysis -
Lenka Roubalová
Status: Ongoing
Specialization:
Molecular mechanisms of the protective effect of natural substances -
Patrik Rulíšek
Status: Ongoing
Specialization: Pathological anatomy and forensic medicine
Computer image analysis in pathology -
Alona Řehulková
Status: Ongoing
Specialization:
Prognostic and predictive factors in solid tumors Cancer cells can be released from primary tumors and transported through the body via blood or lymphatic vessels. These circulating tumor cells (CTCs) can settle and cause the formation of micrometastases in suitable conditions. Numerous studies have shown that CTCs have negative prognostic significance in several types of cancer. CTCs reflect current states of tumors, thus they can provide convenient biomarkers. Furthermore, assessment of their presence and status can facilitate understanding of tumor progression (and/or the development of clinical cancer dormancy), thereby greatly assisting prognostic considerations and therapeutic decisions. Diverse methods are available for detecting, isolating, and characterizing them, which differ in specificity, utility, costs, and sensitivity. We work with RT-qPCR and CytoTrack technology.
Kateřina Sikorová
Status: Ongoing
Specialization:
Genetic variability of bio markers of inflammation in patients with early manifestation of coronary artery disease -
Jan Skácel
Status: Ongoing
Specialization:
Prognostic and predictive markers of ovarian cancer in relation to cytoreductive surgery and hyperthermic intraperitoneal chemotherapy -
Hanuš Slavík
Status: Ongoing
Specialization:
Minimal residual disease in solid cancers The main goal of this thesis is to discover new biomarkers on the level of RNA. These biomarkers can be tested for usage in predictive and prognostic medicine and they can help in early diagnostic or in postoperative identification of the risk of recurrence. The methods used for this purpose are RT-qRCR and microarray assays (cytoscan).
Martin Sněhota
Status: Ongoing
Specialization:
Objectification of quality of ultrasound imaging -
Martina Spisarová
Status: Ongoing
Specialization:
Infulence of chemotherpy and radiotherapy on the immune system -
Jarmila Stanková
Status: Ongoing
Specialization:
Cytotoxic drugs molecular target identification by methods of cell biology and proteomics Molecular target identification of biologically active molecules is a key factor facilitating rational design and preclinical development of potential drugs. Methods of cellular biology, chemical proteomics and mass spectrometry are developed and used for its purposes.
Pavel Stejskal
Status: Ongoing
Specialization:
Circulating tumor cells detection -
Karolína Světlíková
Status: Ongoing
Specialization:
Pathophysiology of selected internal diseases -
Veronika Šlachtová
Status: Ongoing
Specialization:
Design and synthesis of compounds with potential anti-TB properties -
Kateřina Urban
Status: Ongoing
Specialization:
Biomarkers of the risk of developing, effect and toxicity of treatment in gastrointestinal malignancies -
Jan Václavík
Status: Ongoing
Specialization:
Metabolomic tools for diagnosis of inherited metabolic disorders Inherited metabolic disorders of purine and pyrimidine (PP) metabolism exhibit broad neurological, hematological, immunological and renal manifestations. They are characterized by increased concentrations of PP metabolites in body fluids caused by abnormal or missing activity of particular enzyme which is involved in their metabolism. Liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) is method of choice in the last 15 years for diagnoses of PP metabolic disorders. Due to instrumental price drop high‐resolution mass spectrometry (HRMS) is starting to be increasingly used in clinical laboratories. The aim of this work is to compare diagnostic efficacy of these two approaches.
Zuzana Valachová
Status: Ongoing
Specialization:
Predictive and prognostic factors in breast cancer -
David Vanda
Status: Ongoing
Specialization:
Synthesis of heterocyclic compounds as ligands of serotonine receptors and phosphodiesterases -
Monika Vidlařová
Status: Ongoing
Specialization:
Detection and clinical importance of circulating tumor cells in solid tumours The thesis is focused on the detection of circulating tumour cells (CTCs) by different approaches and the clinical importance of their presence in solid tumours, especially in lung, pancreatic and colorectal cancer patients. The main goal is to identify patients with high risk of disease recurrence and in need of adjuvant therapy. The second goal is to participate in the research and development of novel methods of CTCs detection and characterization.
Dominik Vítek
Status: Ongoing
Specialization:
Study of neurodegeneration and aging on model organisms -
Zuzana Vlachova
Status: Ongoing
Specialization:
Prognostic and predictive factors of breast cancer -
Tomáš Zemánek
Status: Ongoing
Specialization:
Prognostic and predictive markers of pancreatic carcinoma -
Ludmila Žárská
Status: Ongoing
Specialization:
Photodynamically active substances possibility of their use for photodynamic therapy of tumors The thesis is focused on the study of effect of various porphyrin and phtalocyanin photosensitizers during different light intensity on cell lines using molecular and microscopic methods.
Vladimír Židlík
Status: Ongoing
Specialization: Pathological anatomy and forensic medicine
The role of cancer microenvironment in prognosis of malignant melanomas -
Patrícia Žižkovičová
Status: Ongoing
Specialization:
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