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Diagnostics of mutations in RAS family oncogenes in tumors using next-generation sequencing
Tumor DNA testing of KRAS and NRAS (RAS) genes, which are components of the EGFR signalling pathway, is a prerequisite for proper personalised biological treatment using anti-EGFR drugs. The main aim of current diploma thesis is to develop a ultra deep amplicon sequencing assay for the detection of somatic mutations in RAS genes. The assay should be robust and functional even for the DNA samples of low quality.