Varanasi Lakshman Ph.D.
Serum is one of several types of patient samples routinely analyzed in the clinic and is often preferred because it can be obtained non-invasively in sufficient quantities. Serum proteins that indicate a condition or disease, its severity, the patient's chances of recovery and of the patient's possible response to therapy are of clinical value. Glycosylation of cellular proteins post-translation has been reported as an indicator of altered cellular physiology. We are currently working on a workflow for the identification of such N-glycosylated proteins using the HCT116 mouse xenograft model system. We are simultaneously exploring ways of scaling up these protocols to increase throughput. The larger goal of the work is to develop marker glycoproteins for the different gastrointestinal cancers.
Publications (Impact Factor Journals):
- OŽDIAN, T., D. HOLUB, Z. MACEČKOVÁ, L. VARANASI, G. RYLOVÁ, J. ŘEHULKA, J. VÁCLAVKOVÁ, H. SLAVÍK, P. MOUDRÝ, P. ZNOJEK, J. STANKOVÁ, J.B. DE SANCTIS, M. HAJDÚCH a P. DŽUBÁK. Proteomic profiling reveals DNA damage, nucleolar and ribosomal stress are the main responses to oxaliplatin treatment in cancer cells. Journal of Proteomics. 2017, 162, 73-85. ISSN 1874-3919 . IF: 3.867. PMID: 28478306
Bachelor students mentor:
status: Ongoing, from: 2018
status: Graduated, from: 2012 to 2013