Koberna Karel CSc.

Koberna Karel CSc.'s picture
Academic Title: RNDr.
Phone: +420 585632184
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EDUCATION

1989 – 1994     Title CSc. (equivalent of PhD), Institute of Experimental Medicine AS CR, Thesis: “Metabolism of Extranucleolar RNA in Architecture of Cell Nucleus”.

1983 – 1988     Title MSc. and RNDr. (Rerum naturalium doctor), Faculty of Science, Charles University in Prague; Specialization – Molecular Biology, Diploma Thesis: “Ultrastructural Immunocytochemical Localization of DNA Replication in Synchronized 3T3 Cells”.

PUBLICATIONS

  1. Ligasová, A., et al. (2016) Open Biol, 6:150172
  2. Ligasová, A., Koberna, K. (2015) Methods Mol. Biol., 1351:81-92, M. McKenzie ed.
  3. Ligasová, A., et al. (2015) PLoS One 10(7): e0132393
  4. Ligasová, A., et al. (2015) PLoS One, 10(2): e0117459
  5. Cmarko, D., et al. (2014) Methods Mol. Biol., 1117:343-66, John Kuo ed.
  6. Ligasová, A., et al. (2013) PLoS One, 8(6):e66864
  7. Ligasová, A., et al. (2012) PLoS One, 7(12):e52584
  8. Liboska, R., et al. (2012) PLoS One, 7(12):e51679
  9. Ligasová, A., et al. (2011) Parasit. Vectors, 4:162
  10. Ligasová, A., Koberna, K. (2011) DNA replication-Current Advances, Intech, H. Seligmann ed.
  11. Ligasová, A., Koberna K. (2010) Nucleic Acids Res., 38(16):e167
  12. Ligasová, A., et al. (2009) J. Struct. Biol., 165(3):204-213
  13. Cvačková, Z., et al. (2008) Chromosoma, 117(5):487-497
  14. Kalmárová, M., et al. (2007) J. Struct. Biol., 160(1):49-56
  15. Cmarko, D., Koberna, K. (2007) Methods Mol. Biol. 369:213-228, J. Kuo ed.
  16. Stavreva, D. A., et al. (2006) Mol. Cell Biol., 26:5131-5145
  17. Smirnov, E., et al. (2006) Folia Biol., 52:59-70
  18. Fidlerová ,H., et al. (2005) J. Cell Biochem., 94:899-916
  19. Pliss, A., et al. (2005) J. Cell Biochem., 15:554-565
  20. Li, J., et al. (2005) EMBO J., 12:120-127
  21. Koberna, K., et al. (2005) J. Cell Biochem., 94(1):126-138
  22. Večeřová, J., et al. (2004) Mol. Biol. Cell, 15:4904-4910
  23. Raška, I., et al. (2004) Biol. Cell, 96:579-594
  24. Malínský, J., et al. (2002) J. Struct. Biol., 140:227-231
  25. Koberna, K., et al. (2002) J. Cell Biol., 157:743-748
  26. Kopský, V., et al. (2002) Fol. Biol. (Prague), 48:69-72
  27. Staněk, D., et al. (2001) Chromosoma, 110(7):460-470
  28. Malínský, J., et al. (2001) J. Cell Sci., 114(Pt 4):747-750
  29. Koberna, K., et al., (2000) Acta Histochem., 102(1):15-20
  30. Melčák, I., et al. (2000) Mol. Biol. Cell., 11(2):497-510
  31. Koberna, K., et al. (1999) Chromosoma, 108(5):325-335
  32. Koberna, K., et al. (1998) Reprod. Nutr. Dev., 38(1):117-126
  33. Schul, W., et al. (1996) EMBO J., 15(11):2883-2892
  34. Raška, I., et al. (1995) J. Struct. Biol., 114(1):1-22
  35. Raška, I., et al. (1992) Cell Biol. Int. Rep., 16(8):771-789
  36. Raška, I., et al. (1989) Exp. Cell Res., 184(1):81-89
  37. Raška, I., et al. (1987) Histochem. J., 19(10-11):603

PATENTS                                      

No. 295681: Method for the delivery of molecules and ions in living cells

No. 302613: Method for the detection of RNA molecules polyadenylated at the 3´ end and RNA molecules containing inner parts composed of several adenines by means of 5-bromo-2 ´-deoxyuridine, 5-iodo-2´-deoxyuridine and 5-chloro-2´-deoxyuridine in the triphosphate form in the permeabilised cells and tissues and on the sections of cells and tissues.

No. 303230: Method for the revelation of specific pyrimidine nucleosides in the structure of the double-stranded DNA for the reaction with antibodies that react with these nucleosides

No. 303251: Method for the labeling of double-stranded DNA molecules

No. 303252: Method for the elimination of antigen attribute of 5-ethynyl-2'-deoxyuridine and 5-ethynyluridine in the detection of nucleic acids

UTILITY MODELS

No. 28964: Kit for accessing specific pyrimidine nucleosides in the structure of two-chain DNA for reaction with antibodies that react with these nucleosides

HONOURS AND AWARDS

2003     Premium of Otto Wichterle for young scientists AS CR


Publications (Impact Factor Journals):

  • [6]
    LIGASOVÁ, A., P. KONEČNÝ, I. FRYDRYCH a K. KOBERNA. Cell cycle profiling by image and flow cytometry: The optimised protocol for the detection of replicational activity using 5-Bromo-2'-deoxyuridine, low concentration of hydrochloric acid and exonuclease III. PLOS One. 2017, 12(4), e0175880. ISSN 1932-6203. IF: 3.057. PMID: 28426799
  • [5]
    LIGASOVÁ, A., P. KONEČNÝ, I. FRYDRYCH a K. KOBERNA. Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication. PLOS One. 2017, 12(3), e0174893. ISSN 1932-6203. IF: 3.057. PMID: 28358913
  • [4]
    LIGASOVÁ, A., R. LIBOSKA, D. FRIEDECKÝ, K. MIČOVÁ, T. ADAM, T. OŽDIAN, I. ROSENBERG a K. KOBERNA. Dr Jekyll and Mr Hyde: A Strange Case of 5-Ethynyl-2'-deoxyuridine and 5-ethynyl-2'-deoxycytidine. Open Biology. 2016, 6: 150172, 1-11. ISSN 2046-2441. IF: 3.481. PMID: 26740587
  • [3]
    LIGASOVÁ, A., R. LIBOSKA, I. ROSENBERG a K. KOBERNA. The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2'-Deoxyuridine in Cellular DNA under Various Conditions. PLOS One. 2015, 10(7), e0132393. ISSN 1932-6203. IF: 3.057. PMID: 26161977
  • [2]
    LIGASOVÁ, A., D. STRUNIN, D. FRIEDECKÝ, T. ADAM a K. KOBERNA. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity. PLOS One. 2015, 10(2), 1-21. ISSN 1932-6203. IF: 3.057. PMID: 25671308
  • [1]
    LIGASOVÁ, A., D. STRUNIN a K. KOBERNA. A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA. PLOS One. 2013, 8(6), 1-6. ISSN 1932-6203. IF: 3.534. PMID: 23825578

Book Chapters:

  • [2]
    LIGASOVÁ, A. a K. KOBERNA. Tracking Mitochondrial DNA In Situ. In: Methods in molecular biology . 3. vyd. United States: Clifton, N.J.: Humana Press, 2016. Kapitola 7, s. 81-92. ISBN: 978-1-4939-3039-5.
  • [1]
    CMARKO, D., A. LIGASOVÁ a K. KOBERNA. Tracking DNA and RNA sequences at high resolution. In: Electron Microscopy Methods in Molecular Biology. 3. vyd. New York: Humana Press, 2014. Kapitola 16, s. 343-366. ISBN: 978-1-62703-775-4.

Patents, Utility models & other IP's :

[4] A METHOD OF STABILIZING ANTIBODIES RECOGNIZING 5-BROMO-2'-DEOXYURIDINE AND/OR 5-CHLORO-2'-DEOXYURIDINE AND/OR 5-IODO-2'-DEOXYURIDINE AT SITES OF INCORPORATION OF THESE NUCLEOSIDES INTO THE DNA AFTER TREATMENT WITH MONOVALENT COPPER AND/OR EXONUCLEASE III, PV 2015-183

Patent: CZ 307 016, Granted: 4. 10. 2017, Ownership:Ownership: Palacký university, Olomouc, Inventors: Koberna Karel, Ligasová Anna

Status: Available

[3] DEVICE FOR MAGNETIC SEPARATION OF FERROMAGNETIC PARTICLES, KIT FOR MAGNETIC SEPARATION OF THE PARTICLES, METHOD OF SEPARATION OF MAGNETIC PARTICLES FROM A SOLUTION AND USE THEREOF, Priority Application PV 2015-141
  1. Patent: CZ 306 187, Granted: 3. 8. 2016, Ownership: Palacky University Olomouc, Inventors:  Koberna KarelLigasová Anna

Status: Available

[2] STAND FOR MAGNETIC SEPARATION OF FERROMAGNETIC PARTICLES, Priority Appl. CZ PUV 2015-40306
  1. Design: CZ 36 681, Granted 2. 3. 2016, Ownership: Palacky University Olomouc, Inventors: Koberna KarelLigasová Anna

Status: Available

[1] KIT FOR MAKING SPECIFIC PYRIMIDINE NUCLEOTIDES IN THE STRUCTURE OF DOUBLE-STANDED DNA TO REACT WITH ANTIBODIES THAT REACT WITH THESE NUCLEOSIDES, Priority Appl. CZ PUV 2015-31075
  1. Utility Model: CZ 28 964, Granted: 14. 12. 2015, Ownership: Palacky University Olomouc, Inventors: Koberna KarelLigasová Anna

Status: Available



Master students mentor:

[1] Zuzana Zálešáková, Testing and optimization of new non-destructive method for determining the activity of cytidine deaminase for use of various cell lines,
status: Graduated, from: 2015 to 2017


Bachelor students mentor:

[1] Jan Moštěk, Base excision repairs,
status: Ongoing, from: 2017