Václavík Jan

Václavík Jan's picture
Academic Title: Ing.
Phone: +420 585632176
Curriculum Vitae: download

I am interested in evaluation of diagnostic performance of high-performance liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) and tandem mass spectrometry (LC-MS/MS) for identification of biomarkers of purine and pyrimidine metabolic disorders and I works on LC-MS method development.

Doctoral training
Metabolomic tools for diagnosis of inherited metabolic disorders
Inherited metabolic disorders of purine and pyrimidine (PP) metabolism exhibit broad neurological, hematological, immunological and renal manifestations. They are characterized by increased concentrations of PP metabolites in body fluids caused by abnormal or missing activity of particular enzyme which is involved in their metabolism. Liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) is method of choice in the last 15 years for diagnoses of PP metabolic disorders. Due to instrumental price drop high‐resolution mass spectrometry (HRMS) is starting to be increasingly used in clinical laboratories. The aim of this work is to compare diagnostic efficacy of these two approaches.


Adam Tomáš Ph.D.



Publications (Impact Factor Journals):

  • [2]
    MÁDROVÁ, L., M. KRIJT, V. BARESOVA, J. VÁCLAVÍK, D. FRIEDECKÝ, D. DOBEŠOVÁ, O. SOUCKOVA, V. SKOPKOVA, T. ADAM a M. ZIKANOVA. Mass spectrometric analysis of purine de novo biosynthesis intermediates. PLOS One. 2018, 13(12), e0208947. ISSN 1932-6203. IF: 2.766. PMID: 30532129
  • [1]
    VÁCLAVÍK, J., K. COENE, I. VROBEL, L. NAJDEKR, D. FRIEDECKÝ, R. KARLÍKOVÁ, L. MÁDROVÁ, A. PETSALO, U. ENGELKE, A. VAN WEGBERG, L. KLUIJTMANS, T. ADAM a R. WEVERS. Structural elucidation of novel biomarkers of known metabolic disorders based on multistage fragmentation mass spectra. Journal of Inherited Metabolic Disease. 2018, 41(3), 407-414. ISSN 0141-8955. IF: 3.970. PMID: 29139026