Fialová Barbora M.Sc.
Phone: +420 585639446
Implications of epigenetic changes in cells derived from prostate cancer
The thesis is focused on a study of DNA methyltransferase (5-aza-2´-deoxycytidine, Aza-dC) and histone deacetylase (sodium butyrate, NaB) inhibitors effects on prostate cancer cells with modified expression of the androgen receptor gene. The androgen-independent human prostate cancer cell lines PC3 (without a functional androgen receptor) and DU145 (with strongly limited expression due to methylation of the androgen receptor gene) are used. The methodology is based on PCR of bisulfite-modified DNA and RT-PCR with bisulfite-sequencing.
Supervisor:Smešný Trtková Kateřina Ph.D.
Status:Ongoing 2009 - 2009
Publications (Impact Factor Journals):
- FIALOVÁ, B., P. LUŽNÁ, J. GURSKÝ, K. LANGOVA, Z. KOLÁŘ a K. SMEŠNÝ TRTKOVÁ. Epigenetic modulation of AR gene expression in prostate cancer DU145 cells with the combination of sodium butyrate and 5'-Aza-2'-deoxycytidine. Oncology Reports. 2016, 36, 2365-2374. ISSN 1021-335X . IF: 2.662. PMID: 27499010
- FIALOVÁ, B., K. SMEŠNÝ TRTKOVÁ, L. PASKOVA, K. LANGOVA a Z. KOLÁŘ. Effect of histone deacetylase and DNA methyltransferase inhibitors on the expression of the androgen receptor gene in androgen-independent prostate cancer cell lines. Oncology Reports. 2013, 29(5), 2039-2045. ISSN 1021-335X. IF: 2.191. PMID: 23503510