Ligasová Anna Ph.D.

Ligasová Anna Ph.D.'s picture
Academic Title: RNDr.
Phone: +420 585632184
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EDUCATION

2002 – 2009     Title PhD, 1st. Faculty of Medicine, Charles University in Prague, Thesis: “DNA Replication and Chromatin: from 3D to Function”.

2009                   Title RNDr., Faculty of Science, Charles University in Prague

1997 – 2002     Title MSc., Faculty of Natural Sciences, Comenius University in Bratislava; Specialization: Cytology; Diploma Thesis: “The Effect of Stimuli and Beta-                               adrenergic Blockers on the Serotonin Release from the Human Blood Platelets in vitro”.

PUBLICATIONS

  1. Ligasová, A., et al. (2017) PLoS One 12(4): e0175880
  2. Ligasová, A., et al. (2017) PLoS One 12(3): e0174893
  3. Ligasová, A., et al. (2016) Open Biol, 6:150172
  4. Ligasová, A., Koberna, K. (2015) Methods Mol. Biol., 1351:81-92, M. McKenzie ed.
  5. Ligasová, A., et al. (2015) PLoS One 10(7): e0132393
  6. Ligasová, A., et al. (2015) PLoS One, 10(2): e0117459
  7. Cmarko, D., et al. (2014) Methods Mol. Biol., 1117:343-66, John Kuo ed.
  8. Ligasová, A., et al. (2013) PLoS One, 8(6):e66864
  9. Ligasová, A., et al. (2012) PLoS One, 7(12):e52584
  10. Liboska, R., et al. (2012) PLoS One, 7(12):e51679
  11. Ligasová, A., et al. (2011) Parasit. Vectors, 4:162
  12. Ligasová, A., Koberna, K. (2011) DNA replication-Current Advances, Intech, H. Seligmann ed.
  13. Ligasová, A., Koberna K. (2010) Nucleic Acids Res., 38(16):e167
  14. Ligasová, A., et al. (2009) J. Struct. Biol., 165(3):204-213
  15. Cvačková, Z., et al. (2008) Chromosoma, 117(5):487-497
  16. Kalmárová, M., et al. (2007) J. Struct. Biol., 160(1):49-56
  17. Koberna, K., et al. (2005) J. Cell Biochem., 94(1):126-138

PATENTS

  • No. 307016: Method of stabilisation of antibodies recognizing  5-bromo-2´-deoxyuridine and/or 5-chloro-2´-deoxyuridine and/or 5-iodo-2´-deoxyuridine at sites of incorporation of these nucleosides into DNA after the acton of monovalent copper ions and/or exonuclease III
  • No. 306187: Apparatus for magnetic separation of ferromagnetic particles, set for magnetic separation of particles, separation process of magnetic particles from a solution and use of the apparatus or set for magnetic separation of particles
  • No. 303230: Method for the revelation of specific pyrimidine nucleosides in the structure of the double-stranded DNA for the reaction with antibodies that react with these nucleosides
  • No. 303251: Method for the labeling of double-stranded DNA molecules
  • No. 303252: Method for the elimination of antigen attribute of 5-ethynyl-2'-deoxyuridine and 5-ethynyluridine in the detection of nucleic acids
  • No. 302613: Method for the detection of RNA molecules polyadenylated at the 3´ end and RNA molecules containing inner parts composed of several adenines by means of 5-bromo-2´-deoxyuridine, 5-iodo-2´-deoxyuridine and 5-chloro-2´-deoxyuridine in the triphosphate form in the permeabilised cells and tissues and on the sections of cells and tissues.

UTILITY MODELS

No. 28964: Kit for accessing specific pyrimidine nucleosides in the structure of two-chain DNA for reaction with antibodies that react with these nucleosides


Publications (Impact Factor Journals):

  • [6]
    LIGASOVÁ, A., P. KONEČNÝ, I. FRYDRYCH a K. KOBERNA. Cell cycle profiling by image and flow cytometry: The optimised protocol for the detection of replicational activity using 5-Bromo-2'-deoxyuridine, low concentration of hydrochloric acid and exonuclease III. PLOS One. 2017, 12(4), e0175880. ISSN 1932-6203. IF: 3.057. PMID: 28426799
  • [5]
    LIGASOVÁ, A., P. KONEČNÝ, I. FRYDRYCH a K. KOBERNA. Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication. PLOS One. 2017, 12(3), e0174893. ISSN 1932-6203. IF: 3.057. PMID: 28358913
  • [4]
    LIGASOVÁ, A., R. LIBOSKA, D. FRIEDECKÝ, K. MIČOVÁ, T. ADAM, T. OŽDIAN, I. ROSENBERG a K. KOBERNA. Dr Jekyll and Mr Hyde: A Strange Case of 5-Ethynyl-2'-deoxyuridine and 5-ethynyl-2'-deoxycytidine. Open Biology. 2016, 6: 150172, 1-11. ISSN 2046-2441. IF: 3.481. PMID: 26740587
  • [3]
    LIGASOVÁ, A., R. LIBOSKA, I. ROSENBERG a K. KOBERNA. The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2'-Deoxyuridine in Cellular DNA under Various Conditions. PLOS One. 2015, 10(7), e0132393. ISSN 1932-6203. IF: 3.057. PMID: 26161977
  • [2]
    LIGASOVÁ, A., D. STRUNIN, D. FRIEDECKÝ, T. ADAM a K. KOBERNA. A fatal combination: a thymidylate synthase inhibitor with DNA damaging activity. PLOS One. 2015, 10(2), 1-21. ISSN 1932-6203. IF: 3.057. PMID: 25671308
  • [1]
    LIGASOVÁ, A., D. STRUNIN a K. KOBERNA. A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA. PLOS One. 2013, 8(6), 1-6. ISSN 1932-6203. IF: 3.534. PMID: 23825578

Book Chapters:

  • [2]
    LIGASOVÁ, A. a K. KOBERNA. Tracking Mitochondrial DNA In Situ. In: Methods in molecular biology . 3. vyd. United States: Clifton, N.J.: Humana Press, 2016. Kapitola 7, s. 81-92. ISBN: 978-1-4939-3039-5.
  • [1]
    CMARKO, D., A. LIGASOVÁ a K. KOBERNA. Tracking DNA and RNA sequences at high resolution. In: Electron Microscopy Methods in Molecular Biology. 3. vyd. New York: Humana Press, 2014. Kapitola 16, s. 343-366. ISBN: 978-1-62703-775-4.

Patents, Utility models & other IP's :

[8] METHOD OF DETERMINING THE ACTIVITY OF ENZYMES CONVERTING CYTOSINE DERIVATIVES TO URACIL DERIVATIVES IN CELLS, TISSUES AND ORGANISMS, PV 2014-579

Patent: EP 3186387; Granted: 17. 10. 2018; Ownership: Palacký University, Olomouc; Institute of Organic Chemistry and Biochemistry AS CR, Prague; Institute of Applied Biotechnologies a.s., Prague; Inventors: Koberna KarelLigasová Anna, Rosenberg, Liboska, Páv, Kvapil, Fišer

Status: Available

[7] DEVICE FOR FACILITATING THE HANDLING OF SOLVENTS, MIXTURES AND SAMPLES ON CARRIERS, SET FOR HANDLING THEM, AND USE THEREOF, PV 2016-820

Patent: CZ 307 454; Granted: 5. 9. 2018; Ownership: Palacký University, Olomouc; Inventors: Koberna KarelLigasová Anna

Status: Available

[6] A METHOD OF DETERMINING THE AMOUNT OF DNA IN SAMPLES, A SET FOR DETERMINING THE AMOUNT OF DNA IN SAMPLES, AND USE THEREOF , PV 2017-233

Patent: CZ 307 415, Granted: 1. 8. 2018, Ownership: Palacký University, Olomouc, Inventors: Koberna KarelLigasová Anna

Status: Available

[5] KIT FOR THE PREPARATION AND THE METHOD FOR THE PREPARATION OF FIXED AND PERMEABILISED MYCOPLASMAS FOR THEIR SUBSEQUENT DETECTION, PV 2016-775

Patent: CZ 307 304, Granted: 23. 5. 2018, Ownership: Palacký University, Olomouc, Inventors: Koberna KarelLigasová Anna

Status: Available

[4] A METHOD OF STABILIZING ANTIBODIES RECOGNIZING 5-BROMO-2'-DEOXYURIDINE AND/OR 5-CHLORO-2'-DEOXYURIDINE AND/OR 5-IODO-2'-DEOXYURIDINE AT SITES OF INCORPORATION OF THESE NUCLEOSIDES INTO THE DNA AFTER TREATMENT WITH MONOVALENT COPPER AND/OR EXONUCLEASE III, PV 2015-183

Patent: CZ 307 016, Granted: 4. 10. 2017, Ownership:Palacký University, Olomouc, Inventors: Koberna Karel, Ligasová Anna

Status: Available

[3] DEVICE FOR MAGNETIC SEPARATION OF FERROMAGNETIC PARTICLES, KIT FOR MAGNETIC SEPARATION OF THE PARTICLES, METHOD OF SEPARATION OF MAGNETIC PARTICLES FROM A SOLUTION AND USE THEREOF, Priority Application PV 2015-141
  1. Patent: CZ 306 187, Granted: 3. 8. 2016, Ownership: Palacky University Olomouc, Inventors:  Koberna KarelLigasová Anna

Status: Available for non-exclusive License 

[2] STAND FOR MAGNETIC SEPARATION OF FERROMAGNETIC PARTICLES, Priority Appl. CZ PUV 2015-40306
  1. Design: CZ 36 681, Granted 2. 3. 2016, Ownership: Palacky University Olomouc, Inventors: Koberna KarelLigasová Anna

Status: Available

[1] KIT FOR MAKING SPECIFIC PYRIMIDINE NUCLEOTIDES IN THE STRUCTURE OF DOUBLE-STANDED DNA TO REACT WITH ANTIBODIES THAT REACT WITH THESE NUCLEOSIDES, Priority Appl. CZ PUV 2015-31075
  1. Utility Model: CZ 28 964, Granted: 14. 12. 2015, Ownership: Palacky University Olomouc, Inventors: Koberna KarelLigasová Anna

Status: Available