Proteomic Facility

 

The proteomics core features a comprehensive set of analytical instruments to cover a range of proteomic analyses. The Orbitrap Elite Hybrid Ion Trap-Orbitrap ETD (Thermo Scientific) for discovery proteomics, the QToF5600 (ABSciex) for SWATH analysis and a QTrap 5500 (ABSciex) for protein quantitation and validation by single/multiple reaction monitoring (SRM/MRM), all coupled to Ultimate 3000 RSLC nano high-performance liquid chromatography systems (Dionex). The facility also has an HCTultra PTM Discovery System (Bruker Daltonics) for PTM analyses and two ToF ultrafleXtreme (Bruker Daltonics) systems for MALDI/NALDI analyses.

A Quadra3 liquid handling workstation (TOMTEC Life Sciences) for solid phase extraction, PROTEINEER dp (Bruker Daltonics) in-gel digestion and MALDI sample preparation station, 1D, 2D, DIGE scanner Typhoon (GE), recombinant protein production and purification instrumentation NGC-Discover Chromatography system (Bio-Rad) is also available to expedite high-volume sample preparation and analysis.

Instrumentation is supported by data management and complex analyses systems that are administered by bioinformatic and IT support staff.

  • Services
    • Protein identification, discovery, quantitation (labelled, label-free), SILAC, FASP
    • Biomarker discovery, validation, SWATH, SRM, MRM, glycocapture
    • Identification of protein post-translational modifications
    • Immunoaffinity pull-down experiments, molecular targets identification
    • Mass spectrometry data analyses,
    • Consultation, training

 

Head of the facility

  • Tomáš Ozdian