Graduated doctoral students

khushboo.agrawal@upol.cz's picture

Khushboo Agrawal

Supervisor: Hajdúch Marián M.D., Ph.D.
Status: Graduated
Specialization:

5-Azacytidine Nucleosides and their Derivatives: Molecular Hallmarks of Drug Resistance & Alternative Therapeutic Regimen

The thesis is mainly focused towards the investigation of the mechanisms of chemotherapeutic resistance to DNA methylation inhibitors. The study will aid to the understanding of the molecular basis of acquired tumor resistance and help in predicting its clinical response, as well as in designing alternative treatment regimens for overcoming resistance.
lucie.borkova@upol.cz's picture

Lucie Borková

Supervisor: Urban Milan Ph.D.
Status: Graduated
Specialization:

Modification of triterpenes with the use of transition metal catalyzed reactions and nucleophilic substitution

In this work, a larger study of transition metal catalyzed reaaction of triterpenes should be studied in order to find a new approach for a preparation of novel derivatives with high cytotoxic activity and favorable pharmacological properties.
alzbeta.kalivodova@upol.cz's picture

Alžběta Gardlo

Supervisor: Hron Karel Ph.D.
Status: Graduated
Specialization:

Multidimensional statistical methods for analysis of human metabolome

The aim of the thesis is to study the possibility of using advanced multivariate statistical methods for statistical analysis of metabolomical data, including the use of appropriate software tools. The main part of the thesis is the application of the methodology of logratio analysis of compositional data.
dusan.holub@upol.cz's picture

Dušan Holub

Supervisor: Džubák Petr M.D., Ph.D.
Status: Graduated
Specialization:

Development of a method for the sensitive and quantitative determination of hepcidin in human serum using LCMS

Hepcidin, a 25 amino acid peptide, plays an important role in iron homeostasis. As such, it has potential as an indicator of any iron-related condition, such as anemia. My research focuses on the measurement of hepcidin in biological fluids to improve our understanding of iron-related disorders. A possible result of such studies could be the development of hepcidin as a tool for diagnosis and clinical management of such disorders.
magdalena.megova@upol.cz's picture

Magdalena Houdová Megová

Supervisor: Trojanec Radek Ph.D.
Status: Graduated
Specialization:

Cytogenetics changes in solid tumors

The thesis is focused on the identification of molecular and genetic changes in solid tumors focusing on glial tumors especially on a group of recurrent glioblastoma. The main goal is to identify molecular and genetic aberrations harboured by glial tumors and selected these which are responsible for glial tumor progression and recurrence.
htoutou.sedlakova@imtm.cz's picture

Miroslava Htoutou Sedlakova

Supervisor: Kolář Milan M.D., Ph.D.
Status: Graduated
Specialization:

Clinical impact of extended-spectrum beta-lactamases

The most frequent mechanism of resistance to beta-lactam antibiotics in Gram-negative bacteria is production of beta-lactamases. Those having serious clinical significance are mainly ESBL and AmpC broad-spectrum beta-lactamases and, recently, carbapenemases. For adequate antibiotic therapy of infections caused by producers of these enzymes, it is necessary to not only rapidly and correctly identify the etiological agents and their susceptibility to antibiotics but also detect the presence of these dangerous phenotypes of resistance. One of the objectives of this dissertation was to determine the sensitivity of phenotypic methods for detecting broad-spectrum beta-lactamases and carbapenemases and to select the most suitable ones for routine microbiology practice.
katarina.chroma@upol.cz's picture

Katarína Chromá

Supervisor:
Status: Graduated
Specialization:

Using novel anti-cancer agents in elucidation of DNA damage response (DDR) mechanisms and their role in onset of cancer

The thesis is centered on cellular responses, termed DNA damage response (DDR) to DNA double-strand breaks, presumably the most deleterious lesions affecting DNA. The aim of the thesis is to shed more light on certain mechanistic aspects of the DDR. To this end, several chemical inhibitors interfering with different key steps of the DDR are utilized. Apart from deciphering the underlying mechanisms, this approach might lead to new diagnostic and therapeutic approaches.
sylwia.jancik@upol.cz's picture

Sylwia Jančík

Supervisor: Drábek Jiří Ph.D.
Status: Graduated
Specialization: Pediatrics

New strategies for genotyping of KRAs and BRAF onkomarkesrs in heterogeneous clinical tumors samples

The thesis is focused on genotyping of KRAS and BRAF in heterogeneous clinical tumor samples using different molecular genetics methods (Sanger direct sequencing, COLD-PCR, CE-IVD PCR Real-Time methods, and massively parallel sequencing).
radana.karlikova@upol.cz's picture

Radana Karlíková

Supervisor: Friedecký David Ph.D.
Status: Graduated
Specialization:

Study of patobiochemistry of inherited metabolic disorders by metabolomic approach

The thesis is focused on the targeted and untargeted metabolomic analysis of selected diseases by liquid chromatography coupled with tandem mass spectrometry and high resolution mass spectrometry. The main goal is the identification of biomarkers and understanding changes in metablolic pathways important for patobiochemistry of inherited disorders.
petr.konecny07@upol.cz's picture

Petr Konečný

Supervisor: Džubák Petr M.D., Ph.D.
Status: Graduated
Specialization:

Screening of chemical compounds´ biological activities in vitro

The thesis is focused on the identification of potentially active anticancer, antibacterial and antiparasitic compounds. Using flow cytometric evaluation provides information about cell cycle modifications after treatment with different chemical compounds and allows selection of most potent agents for further testing or synthesis of more efficient analogues.
jakub.malohlava@upol.cz's picture

Jakub Malohlava

Supervisor: Bartoň Tománková Kateřina Ph.D.
Status: Graduated
Specialization:

Study of photodynamically active substances using modern microscopic techniques

The thesis is focused on the evaluation of substances used for inducing photodynamic effect in cancerous cells. The main aim is to assess the effect on mechanical properties using atomic force microscopy.
katerina.micova01@upol.cz's picture

Kateřina Mičová

Supervisor: Friedecký David Ph.D.
Status: Graduated
Specialization:

Significance of intracellular and plasma levels of new tyrosine kinase inhibitors

The thesis is focused on development of HPLC-MS/MS methods for determination of tyrosine kinase inhibitors imatinib, nilotinib and dasatinib used for the treatment of chronic myeloid leukemia, and also on relationship between imatinib plasma levels and treatment response.
lukas.najdekr@upol.cz's picture

Lukáš Najdekr

Supervisor: Adam Tomáš Ph.D.
Status: Graduated
Specialization:

Metabolomic tools in diagnosis of inborn errors of metabolism

The thesis is focused on untargeted metabolomics methods (high resolution exact mass spectrometry (HRMS) based methods coupled with liquid chromatography) and their development together with statistical evaluation tools. Final application will be used to study metabolomic changes in biological materials (dry blood spots, plasma, cell extracts).
lucie.navratilova@upol.cz's picture

Lucie Navrátilová

Supervisor: Raclavský Vladislav M.D., Ph.D.
Status: Graduated
Specialization:

Identification and typing of nonfermenting gram-negative rods recovered from lungs of cystic fibrosis and chronic osbstructive pulmonary disease (COPD) patients

The thesis is focused on the developent of novel approaches to identification and typing of bacterial pathogens combining the technique of PCR and high-resolution melting analysis (HRMA) with the aim to translate the advances of economic and rapid molecular techniques into routine diagnostics in favor of best tailored antimicrobial therapy in chronic lung diseases.
tomas.ozdian@upol.cz's picture

Tomáš Oždian

Supervisor: Džubák Petr M.D., Ph.D.
Status: Graduated
Specialization:

Cancer proteomics in clinical and experimental oncology

The thesis is focused on the detection possible biomarkers of cancer treatment efficiancy. Main task is to prepare database of proteomic profiles of current clinically available anticancer drugs.
klara.pizova@upol.cz's picture

Klára Pížová

Supervisor: Kolářová Hana Ph.D.
Status: Graduated
Specialization:

Effect of electromagnetic radiation and ultrasound on cell lines

The thesis is focused on in vitro testing of different sensitizers for photodynamic and sonodynamic treatment. The main goal is to identify the best sensitizer concentrations and total radiation doses and identify mechanisms of their action.
zuzana.matuskova@upol.cz's picture

Zuzana Rácová (Matušková)

Supervisor: Anzenbacher Pavel D.Sc.
Status: Graduated
Specialization:

Cytochromes P450 - main enzymes in drug metabolism: Study of properties of these enzymes in rat gastrointestinal tract and their influence by probiotics

The study is focused on effect of probiotics on cytochromes P450 and pharmacokinetics of concomitatntly taken drug.
veronika.fulopova@upol.cz's picture

Veronika Ručilová

Supervisor: Soural Miroslav Ph.D.
Status: Graduated
Specialization:

Development of Strategies for Solid-Phase Synthesis of Nitrogenous Heterocyclic Compounds Based on the 2- and 4-Nitrobenzenesulfonamide Chemistry

The submitted doctoral thesis deals with the development of simple procedures applicable for the preparation of libraries of selected heterocyclic compounds. In all of the introduced procedures, the immobilized 2- or 4-nitrobenzenesulfonamides were used as the key intermediates. Different scenarios have been used in which the nitrobenzenesulfonyl (Nos) group was applied (1) only as a protective/activating group for Fukuyama selective monoalkylation followed by the cleavage of the sulfonamide scaffold, (2) as a building block after previous Fukuyma alkylation, or (3) as a building block without application of Fukuyama alkylation. The key intermediates were further modified and converted to the target products under different cyclization conditions. During our long-term research we have developed high-throughput solid-phase syntheses of various heterocyclic compounds involving six types of different scaffolds.
jiri.rehulka@upol.cz's picture

Jiří Řehulka

Supervisor: Džubák Petr M.D., Ph.D.
Status: Graduated
Specialization:

Mitochondria as a therapeutic target in cancer

Mitochondria are organelles involved in cellular energy metabolism. This aspect of the cell-physiology is often altered during neoplastic transformation/carcinogenesis. For this reason, mitochondria constitute potential targets in cancer therapy. My work focuses on the anti-cancer activity of novel derivatives of betulinic acid in the context of drug-discovery.
anna.stahelova@upol.cz's picture

Anna Sťahelová

Supervisor:
Status: Graduated
Specialization:

Genetic factors in complex diseases with inflammatory compound in pathogenesis

The thesis is focused on the genetic association studies (GAS) in complex diseases. GAS use the comparison of genetic markers (Single Nucleotide Polymorphisms or Copy Number Variations) between the patients with complex diseases and healthy controls. The main goal is to detect susceptibility genes responsible for heritable component of complex diseases.
dana.simkova@upol.cz's picture

Dana Šimková

Supervisor: Bouchal Jan Ph.D.
Status: Graduated
Specialization:

Study of invasive growth and its blocation possibilities in breast and prostate cancer

Thesis is focused on an identification of asporin‘s (protein interacting with extracellular matrix) role in motility and invasiveness of model cell lines. The main goal is to determine if asporin could be considered as a marker of tumor metastatic behaviour.
michaela.svachova@upol.cz's picture

Michaela Šváchová

Supervisor: Bouchal Jan Ph.D.
Status: Graduated
Specialization:

Importance of molecular markers for cancer diagnostics

The thesis is focused on the detection of cancer associated markers. Emphasis is on importance of polymerase chain reaction based gene rearrangements in the diagnosis of lymphoproliferative disorders in biopsy samples. These results have confirmed that the PCR-based analysis is suitable as a routine method and is helpful in establishing a diagnosis in morphologically unclear cases.
renata.vecerova@fnol.cz's picture

Renata Večeřová

Supervisor: Kolář Milan M.D., Ph.D.
Status: Graduated
Specialization:

Možnosti testování antimikrobní aktivity stávajících a nově vyvíjených látek

Cílem práce je definovat antimikrobní účinky nových látek, především nanočástic stříbra. Bude testován účinek na standardní referenční bakteriální kmeny, multirezistentní bakterie a na vybrané kvasinky. Na základě výsledků budou analyzovány potencionální praktické možnosti těchto látek.
ivo.vrobel@upol.cz's picture

Ivo Vrobel

Supervisor: Friedecký David Ph.D.
Status: Graduated
Specialization:

Influence of certain cancerostatics on the cell metabolome

The influence of chosen cancerostatics (TKI, MTX, …) on the cell metabolome is evaluated using LC-MS. The main goal is to find new biomarkers of the treatment response and new drug metabolites using targeted and untargeted metabolomic approach.