Graduated bachelor students

Adámek Richard's picture

Richard Adámek

Bachelor training

Supervisor:
Status: Graduated 2013 to 2016
Modification of triterpenes with the use of selected transition metal catalyzed reactions
The main aim is to prepare a small set of new triterpenoid derivatives using transition metal catalyzed crosscouplings of triterpenes with small molecules. The reaction conditions must be found and then, the new derivatives will be studied for their cytotoxic activity.
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Hana Adámková

Bachelor training

Supervisor:
Status: Graduated 2014 to 2015
Imaging of glioblastoma multiforme in a mouse model using PET/CT system
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Ivana Družbíková

Bachelor training

Status: Graduated 2013 to 2016
Principles of DNA damage
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Dominika Flašarová

Bachelor training

Supervisor:
Status: Graduated 2015 to 2016
Gallium-68 labelled RGD peptides for PET imaging of glioblastoma multiforme
RGD peptides are accumulated in tumors expressing αvβ3 integrin (marker of angiogenesis). This thesis will include radiolabelling of selected RGD peptides. Peptide derivatives will be passed to in vitro tests employing cell line U-87 MG (glioblastoma multiforme) to assess their ability to bind to tumor cells. Finally the appropriate candidates will be used for in vivo imaging of glioma in mouse model. The imaging will be carried out in microPET/CT system and its results will be compared with ex vivo biodistribution study.
Fryčová Gabriela's picture

Gabriela Fryčová

Bachelor training

Status: Graduated 2013 to 2016
Methylome profile changes in cancer cells associated with drug resistance
Aberrant regulation at epigenetic level can directly and/or indirectly influence the genetic integrity and gene expression pattern of cells, resulting in the development of various types of disorders, including cancer. The local hypermethylation of tumor-suppressor genes and the global hypomethylation of genomic DNA often occur in human cancers. Multiple DNA methylation changes in the cancer methylome could be associated with the acquisition of drug resistance. Recently at our institute the cancer cell lines resistant to 2‘-deoxy 5-azacytidine (decitabine) were prepared. Decitabine, a demethylation drug, is used in clinical practice for treatment of hematological malignancies. Since epigenetic therapy is in clinical use or trials for several cancers, efficient methods for epigenetic profiling are needed. Our specific aim is to screen a genome-wide methylation profile of 2-deoxy 5-azacydine resistant cancer cell line HCT116. Our long-term aim is to elucidate the mechanism of drug resistance in cancer cells.
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Karel Fusek

Bachelor training

Supervisor:
Status: Graduated 2015 to 2017
Snapback PCR
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Jiří Hodoň

Bachelor training

Supervisor:
Status: Graduated 2012 to 2015
Attempt to find the mechanizm of action in cytotoxic active triterpenes
Attempt to find the mechanizm of action in cytotoxic active triterpenes . The main goals of this work is to prepare a set of triterpenoid derivatives that have shown high selective cytotoxicity and synthesize biotinylated probes from them that may be used in pull down essays in order to try to find molecular targets.
Horáčková Veronika's picture

Veronika Horáčková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2016
Prevalence of HPV Infection in Women and Man Treated for Infertility
Horka Vladimír's picture

Vladimír Horka

Bachelor training

Supervisor:
Status: Graduated 2016 to 2018
Conjugates of betulin prepared by cycloaddition reactions
miroslav.horvath01@upol.cz's picture

Miroslav Horváth

Bachelor training

Supervisor:
Status: Graduated 2016
Preparation of five condensed rings 1,2,4-triazine containing indole and benzimidazole skeleton
Chlubnová Markéta's picture

Markéta Chlubnová

Bachelor training

Supervisor:
Status: Graduated 2016 to 2017
The anticancer activity mediated by new A3 receptor agonists
The aim of the thesis is to summarize current knowledge about the role of A3 adenosine receptor in cancer development and anti-cancer effects of newly characterized A3 agonists. The experimental part of thesis is focused on whether or not the anti-cancer effects of A3 agonists described in literature are mediated by direct modulation of A3 receptor.
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Nikola Jakubcová

Bachelor training

Supervisor:
Status: Graduated 2016 to 2018
Preparation of substituted thiazole derivatives of ursolic acid
Jenčíková Barbora's picture

Barbora Jenčíková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2015
Prognostic and predictive significance of matrix metalloproteinases in patients with colorectal cancer
Kadlčíková Dominika's picture

Dominika Kadlčíková

Bachelor training

Supervisor:
Status: Graduated 2015 to 2017
Melting curve at microsatellite amplicons
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Pavlína Kalábová

Bachelor training

Supervisor:
Status: Graduated 2013 to 2014
The validation of differentially expressed proteins in CCRF-CEM cell line treated by oxaliplatin
The aim of this thesis is to validate protein changes which is responsible for oxaliplatin treatment. These changes has been previously analyzed by quantitative proteomics. The main techniques used in this bachelory thesis are western blot and classical microscopy.
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Lucie Kotková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2015
Molecular and genetic changes in glial tumors
Kučerková Michaela's picture

Michaela Kučerková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2014
Study of pharmacokinetics selected radiopharmaceuticals in animal models using imaging methods
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Tereza Lausová

Bachelor training

Supervisor:
Status: Graduated 2016 to 2018
MiRNA and mRNA Profiling in Meningiomas
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Kateřina Lónová

Bachelor training

Status: Graduated 2015 to 2016
Development and basic characterization of the cell line resistant to the CDK inhibitors
Bachelor thesis will be focused on the preparation of the resistant clones towards the class of the CDK inhibitors which are used in the cancer treatment and are used in the clinic or in the clinical trials. We plan to use different approaches how to reach the resistant population. After the selection of the resistant cells, the resistant population will be tested for the proliferation characteristics and cross-resistance and general mechanisms of the resistance. Part of the thesis will be summary reviewing currently known mechanisms of the CDKi resistance.
Martinková Júlia's picture

Júlia Martinková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2016
Forms of HPV infection in women at risk for cervical carcinoma
Michalčíková Tereza's picture

Tereza Michalčíková

Bachelor training

Supervisor:
Status: Graduated 2016 to 2017
Labelled peptides for multimodal in vivo imaging of cancer
The aim of this bachelor thesis is preclinical testing of two peptide bioconjugates as potential agents with nuclear and optical signaling for oncological applications. The main goal of the study is to determine in vitro characteristics of studied bioconjugates, their in vivo behaviour in tumor mice and to evaluate their potential for diagnosis of cancer using imaging techniques
Mikolajková Denisa's picture

Denisa Mikolajková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2015
Development of high throughput screening assay for detection of E2F mediated signaling pathways
E2Fs are transcription factors which regulate the cell cycle by different mechanism. E2F family contains eight members E2F1 – E2F8. Some are able to activate and some repress gene expression. Disruption E2Fs signaling pathways is one of the reason cancer diagnosis. Theoretical part summarizes basic information about E2Fs, the cell cycle with its control by cyclin dependent kinases. Also is focused on retinoblastoma protein pRb and drugs used for high throughput screening assay. In experimental part were tested three types of cell clone 293H which were kept in 10% EMEM medium. I chose one clone with the best properties and used it for testing anti cancer drugs by high throughput screening.
Míšek David's picture

David Míšek

Bachelor training

Supervisor:
Status: Graduated 2015 to 2016
Expression of matrix metalloproteinases in tubular adenomas compared to expression in colorectal carcinomas
Olejníková Denisa's picture

Denisa Olejníková

Bachelor training

Supervisor:
Status: Graduated 2016 to 2018
Synthesis of new lupane derivatives modified at position 20 or 30
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Tomáš Oliva

Bachelor training

Status: Graduated 2012 to 2013
Serum-based Markers of Gastrointestinal Cancer
The larger goal of the work is to identify protein indicators of neoplastic diseases of the gut in serum. The xenograft mouse is used as the model system and mouse serum is analyzed using mass spectrometry. These physiological cues are expected to enable a more accurate prognosis of gastrointestinal cancer than is currently possible.
katerina.opravilova01@upol.cz's picture

Kateřina Opravilová

Bachelor training

Status: Graduated 2014 to 2016
The synthesis of selenadiazoles derived from 2,3-dihydroquinoline-4-on
Pěntoň Marek's picture

Marek Pěntoň

Bachelor training

Status: Graduated 2012 to 2015
The design of molecular libraries for massively parallel signature sequencing
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Sebastian Reichel

Bachelor training

Status: Graduated 2014 to 2018
Detection of circulating tumor cells
Ručilová Veronika Ph.D.'s picture

Veronika Ručilová

Bachelor training

Supervisor:
Status: Graduated 2006 to 2009
Solid-Phase synthesis of precursors of bisheterocyclic derivatives of 3-hydroxy-4(1H)-quinolinone.
The main target of this work was preparation of the precursors leading to the new type of 3-hydroxy-4-(1H)-quinolinone derivatives, compounds with potential biological importance. In this regard, we first focused our attention on the synthesis of intermediates resulting in the connection of the heterocycle and hydroxyquinoline skelet in position 7 via spacer with amide group. For this purpose we prepared a model intermediate methyl 4-((3-hydroxypropyl)carbamoyl)-2-nitrobenzoate using the solid-phase synthesis. Later, we worked on the successful conversion of hydroxy group of model intermediate to primary or secondary amino group. After synthesis of target intermediate, we tried to prepare a model bisheterocycle of hydroxyquinoline-thiazole. Unfortunately, the attempt was not successful and it will be necessary to focus on optimisation of the final reaction step.
Stanková Jarmila's picture

Jarmila Stanková

Bachelor training

Supervisor:
Status: Graduated 2014
Determination of the efficacy of 5-fluorouracil on line CCRF-CEM T
Štěpánková Jana's picture

Jana Štěpánková

Bachelor training

Supervisor:
Status: Graduated 2014 to 2015
Preclinical development of 68Ga labelled peptides for glioblastoma imaging
This work is focused on preclinical testing of selected peptides labelled with 68Ga as potential radiodiagnostics for glioblastoma multiforme. The main goal of the study is to determine in vitro characteristics of studied peptides, their in vivo behaviour in mice and to evaluate the potential of 68Ga labelled peptides for diagnosis of glioblastoma multiforme using positron emisson tomography.
Tinková Eva's picture

Eva Tinková

Bachelor training

Status: Graduated 2011 to 2015
MirRNA expression profiling in Barrett’s oesophagus
The main objective of the study is to profile miRNA expression in patients with Barrett’s oesophagus specific novel predictive and prognostic biomarkers using modern microarray technology.
daniel.toman01@upol.cz's picture

Daniel Toman

Bachelor training

Supervisor:
Status: Graduated 2014 to 2016
Synthesis of pyrazol-3(5)-carboxylic acid derivatives
Trapani Patricia's picture

Patricia Trapani

Bachelor training

Supervisor:
Status: Graduated 2015 to 2016
Synthesis of benzothiadiazepines using solid-phase synthesis
Trhlíková Zdeňka's picture

Zdeňka Trhlíková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2017
Preparation of heterocyclic triterpenes
The main aim is to prepare a small set of new heterocyclic triterpenoid derivatives using condensation reactions of triterpenoid precursors with ethylene diamine, urea derivatives, hydrazine and other small molecules. Our previous research of triterpenoid heterocycles resulted in several compounds with high in vitro cytotoxic activity and this work should further explore this topic. New derivatives prepared within this work will be tested for their in vivo cytotoxic activity and the best candidates will be selected for more biological examinations.
Umlaufová Eva's picture

Eva Umlaufová

Bachelor training

Supervisor:
Status: Graduated 2015 to 2016
68Ga-siderophores of Pseudomonas species
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Anna Vávrová

Bachelor training

Status: Graduated 2015 to 2017
Detection and characterization of bacteria producing beta-lactamases isolated from patients with leukemia
The aim of the thesis is the detection and characterization of bacteria producing beta-lactamases isolated from patients with leukemia. All isolates of enterobacteria will be tested for the presence of blaTEM, blaSHV and blaCTX-M genes (responsible for extended-spectrum beta-lactamase production) using PCR method and the eventual occurrence of genes enabling carbapenemases production (KPC, SME, IMI, VIM, IMP, OXA enzyme types etc.) will be assessed. For direct detection of extended-spectrum beta-lactamase (ESBL) producers, new real-time PCR assay will be performed.
Vepřík Pavel's picture

Pavel Vepřík

Bachelor training

Status: Graduated 2012 to 2015
Solid-phase synthesis of dihydropteridine derivatives
Vlčková Adéla's picture

Adéla Vlčková

Bachelor training

Supervisor:
Status: Graduated 2013 to 2014
Studium farmakokinetiky 68Ga značených sideroforů v laboratorních zvířatech
Vránová Barbora's picture

Barbora Vránová

Bachelor training

Supervisor:
Status: Graduated 2016 to 2018
Preparation of betulinic acid derivatives via Suzuki cross-coupling
Vysloužilová Lenka's picture

Lenka Vysloužilová

Bachelor training

Status: Graduated 2013 to 2016
High-throughput sequencing and its use for identifying inherited causes of cardiomyopathies
Cardiomyopathies are illnesses of myocardium connected to myocardial dysfunction. Some are connected to high risk of life treating arrhythmia and already first manifestation could end in sudden death. The most frequent type of cardiomyopathy is dilatation cardiomyopathy (DM). In many cases the causative of disease is unknown. In part of the patients, the inheritance of genetic variation or mutation could play a role in pathogenesis. The aim of this works is the study and identification of inherited forms of DM using high-throughput sequencing on Illumina MiSeq platform.